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Electrochemical L-lysine sensor based on graphene material and preparation method thereof

A technology of lysine oxidase and graphene is applied in the field of L-lysine electrochemical sensor and its preparation, which can solve the problems of no electrochemical activity, complicated analysis process, long detection time, etc., and achieves accelerated direct electron transfer. , large specific surface area, good electrical conductivity

Pending Publication Date: 2018-05-18
SHAOGUAN COLLEGE
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Problems solved by technology

However, due to factors such as structural similarity of amino acids, spectral inertness, weak chromophores, and no electrochemical activity, it brings great challenges to the detection
Most of the existing methods are for the simultaneous detection of multiple or even more than ten kinds of amino acids in tea, fruits and vegetables. The detection time is long, the analysis process is complicated, and there is a bottleneck in the selective identification and detection of a single amino acid.

Method used

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  • Electrochemical L-lysine sensor based on graphene material and preparation method thereof

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Embodiment 1

[0030] see figure 1 , which is a schematic flow chart of preparing a graphene-based L-lysine electrochemical sensor in this embodiment.

[0031] In this example, the L-lysine oxidase produced by Rheinheimera sp. is used to modify the working electrode, and the amino-modified ferrocene / graphene oxide nanocomposite is used as the sensitizing material to construct a graphene-based material through a three-electrode system. L-lysine electrochemical sensor.

[0032] The preparation method of the L-lysine electrochemical sensor based on graphene material specifically comprises the following steps:

[0033] (1) Using 20mg of graphene oxide and 50mg of aminated ferrocene as raw materials, through the coupling of 19.2mg of EDC and 11.5mg of NHS, through the steps of ammonolysis, hydrazine hydrate reduction, washing, and vacuum drying, the amino-modified ferrocene was prepared. Iron / graphene oxide nanocomposite (labeled NF-GO). Next, the NF-GO composite material was dispersed in a DM...

Embodiment 2

[0046] In this example, the L-lysine oxidase extracted from Mucus of Scomber japonicus is used to modify the working electrode, and the indium trioxide / graphene nanocomposite is used as the sensitizing material to construct a graphene-based L - Lysine electrochemical sensor.

[0047] The preparation method of the L-lysine electrochemical sensor based on graphene material specifically comprises the following steps:

[0048] (1) 20mg graphene oxide, 70mg indium acetylacetonate, 150mg sodium hydroxide are added to 10ml volume ratio and be the ethanol of 1:0.8:1:0.4: water: cyclohexane: in the mixed solvent of oleic acid, then in React at 200°C for 20 hours to prepare indium hydroxide / graphene nanocomposite material, then calcinate indium hydroxide / graphene nanocomposite material at high temperature to obtain diindium trioxide / graphene nanocomposite material (marked as IO-GR) . Next, take the IO-GR composite material and disperse it in a DMF solvent, and disperse it ultrasonical...

Embodiment 3

[0060] In this example, the L-lysine oxidase produced by Trichoderma viride is used to modify the working electrode, and the fluorine-substituted graphene nanomaterial is used as the sensitizing material, and the graphene-based L-lysine electrochemical reaction is constructed through a three-electrode system. sensor.

[0061] The preparation method of the L-lysine electrochemical sensor based on graphene material specifically comprises the following steps:

[0062](1) Using fluorinated graphite with a substitution ratio of 0.25 as raw material, fluorinated graphene oxide was prepared by the improved Hummers method, and then prepared by reduction with sodium borohydride and hydrazine hydrate to obtain fluorine-substituted graphene nanomaterials (marked as F 0.25 -GR). Next, take F 0.25 - The GR composite was dispersed in DMF solvent and ultrasonically dispersed for 20 minutes to make F at a concentration of 1 mg / ml 0.25 The DMF solution of -GR is ready for use.

[0063] (2)...

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Abstract

The invention relates to an electrochemical L-lysine sensor based on a graphene material and a preparation method thereof. The preparation method comprises the following steps: modifying a glassy carbon electrode with L-lysine oxidase, with the graphene material as a sensitizing material; and separately arranging the modified glassy carbon electrode used as a working electrode, a saturated calomelelectrode used as a reference electrode and a platinum electrode used as an auxiliary electrode in an electrolytic bath to form a three-electrode system so as to prepare the electrochemical L-lysinesensor. The invention also relates to application of the electrochemical L-lysine sensor based on the graphene material to detection of L-lysine in food or medicines. The electrochemical L-lysine sensor based on the graphene material has the advantages of simple and rapid detection, high sensitivity and low cost and can achieve the purpose of high-selectivity identification and detection of L-lysine.

Description

technical field [0001] The invention relates to the technical field of electrochemical sensors, in particular to a graphene-based L-lysine electrochemical sensor and its preparation method and application. Background technique [0002] Amino acid detection is of great significance in the fields of food processing, nutritional analysis, medical testing, medical diagnosis and scientific research. Most of the existing amino acids in tea or fruits and vegetables are detected by chromatography or automatic amino acid analyzers. Gas chromatography and gas-mass spectrometry were used to determine the free amino acids in tea; or the Hitachi L-8800 amino acid automatic analyzer was used to analyze the amino acid components in the leaves of mature plants of Camellia japonica and Chrysanthemum japonica; some researchers also used amino acid analyzers to determine The contents of 17 kinds of amino acids in the fruits of 5 carambola varieties provide a basis for the selection and breedi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/327G01N27/48
CPCG01N27/308G01N27/3278G01N27/48
Inventor 程金生
Owner SHAOGUAN COLLEGE
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