Zoom optical tweezers two-photon microscopy imaging device and method

Abstract

The invention provides a zooming optical tweezers dual-photon microscopic imaging device and a method and belongs to the technical field of optical microscopic imaging and optical manipulation. According to the technical characteristics, the device comprises a dual-photon illumination module, a dual-photon scanning module, a dual-photon detection module, an optical tweezers focusing module and anoptical tweezers axial focusing module. According to the zooming optical tweezers dual-photon microscopic imaging device, an axial focusing device composed of a polarizing spectroscope, a quarter waveplate, a low aperture objective lens, a tube mirror and a plane reflecting mirror is additionally arranged in a conventional optical tweezers microscopic system, and axial movement of an optical tweezers focal plane in a common optical path optical tweezers dual-photon microscope is realized, so that a suspension sample is grasped to realize axial movement and dual-photon three-dimensional tomographic scanning imaging is realized. The zooming optical tweezers dual-photon microscopic imaging device has the advantages of simplicity in installation and adjustment, rapid zooming and axial tomography speed and low observation cost.

Description

technical field [0001] The invention relates to a microscopic imaging device and method, in particular to a zoom optical tweezers two-photon microscopic imaging device and method, which can realize the separation and adjustment of the common optical path optical tweezers microsystem, and complete three-dimensional two-photon scanning imaging, which belongs to optical Microscopic imaging and optical manipulation technology. Background technique [0002] In conventional objective-type optical tweezers microscopes, the optical tweezers device and the imaging device are usually located on both sides of the sample, which is convenient for separate focusing. However, in some microscopic observation applications that require the introduction of other environmental variables (such as adding radiation The device is used to study the response mechanism of living cells to radiation), and the optical tweezers and the imaging device are required to be located on the same side of the samp...

AI Technical Summary

Problems solved by technology

[0002] In conventional objective-type optical tweezers microscopes, the optical tweezers device and the imaging device are usually located on both sides of the sample, which is convenient for separate focusing. However, in some microscopic observation applications that require the introduction of other environmental variables (such as adding radiation The device is used to study the response mechanism of living cells to radiation), and the optical tweezers and the imaging device are required to be located on the same side of the sample. scanning imaging

Adding a zoom lens or DMD to the optical tweezers system can change the focus position of the optical tweezers to solve the above problems, but the modulation speed is slow or expensive

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