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Zoom two-photon optical tweezers microscopic imaging device and method

A microscopic imaging, two-photon technology, applied in microscopes, measuring devices, fluorescence/phosphorescence, etc., can solve the problems of high cost, difficult separation of focal planes, slow modulation speed, etc., to reduce observation costs, improve zoom and axial Chromatography speed, simple installation and adjustment

Inactive Publication Date: 2019-07-12
HARBIN INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0002] In conventional objective-type optical tweezers microscopes, the optical tweezers device and the imaging device are usually located on both sides of the sample, which is convenient for separate focusing. However, in some microscopic observation applications that require the introduction of other environmental variables (such as adding radiation The device is used to study the response mechanism of living cells to radiation), and the optical tweezers and the imaging device are required to be located on the same side of the sample. scanning imaging
Adding a zoom lens or DMD to the two-photon microscopy system can change the focus position of the two-photon microscopy system to solve the above problems, but the modulation speed is slow or expensive

Method used

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  • Zoom two-photon optical tweezers microscopic imaging device and method
  • Zoom two-photon optical tweezers microscopic imaging device and method
  • Zoom two-photon optical tweezers microscopic imaging device and method

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Embodiment 1

[0031] Embodiment 1: as attached figure 1 The present embodiment shown provides a zoom two-photon optical tweezers microscopic imaging device, which is used to quickly switch the axial position to realize three-dimensional tomographic scanning.

[0032] A zoom two-photon optical tweezers microscopic imaging device, including a two-photon illumination module, a two-photon axial focusing module, a two-photon scanning module, a two-photon detection module and an optical tweezers focusing module:

[0033] According to the light propagation direction, the two-photon lighting module is as follows: laser device 1, beam expander 2, and polarization beam splitter 3;

[0034] According to the light propagation direction, the two-photon axial focusing module is as follows: polarizing beam splitter 3, quarter wave plate 4, objective lens 1 5 and plane mirror 6;

[0035] According to the light propagation direction, the two-photon scanning module is as follows: scanning galvanometer 7, sc...

Embodiment 2

[0047] Embodiment 2: as attached figure 1 and figure 2 The illustrated embodiment provides a zoom two-photon optical tweezers microscopic imaging method, which is used to quickly switch the axial position to realize three-dimensional tomographic scanning.

[0048] A zoom two-photon optical tweezers microscopic imaging method, the method is realized based on the zoom two-photon optical tweezers microscopic imaging device described in embodiment 1, specific steps:

[0049] Step a, laser device one 1 emits excitation light, forms parallel light after passing through beam expander 2 and polarizing beam splitter 3, and parallel light passes through quarter-wave plate 4 and objective lens one 5 and is transmitted to plane mirror 6 to generate reflected laser light, and then After the scanning galvanometer 7, scanning lens 8, tube mirror one 9, dichroic mirror one 10, dichroic mirror two 11 and objective lens two 12 transmit, a focused spot is formed on the sample 13 to be tested, ...

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Abstract

The invention relates to a zoom two-photon optical tweezers microimaging device and method, and belongs to the technical fields of optical microimaging and optical manipulation. The patent for invention has the following technical characteristics: the device comprises a two-photon lighting module, a two-photon scanning module, a two-photon detection module, a two-photon axial focusing module and an optical tweezers focusing module. An axial focusing device consisting of a polarizing beam splitter, a quarter-wave plate, a low-aperture objective lens, a tube mirror and a plane reflecting mirroris increased in the conventional two-photon microscopic system, and axial movement of the two-photon focal plane of a common-path optical tweezers two-photon microscope is realized, so that axial movement of grabbing a suspending sample is realized and two-photon three-dimensional tomographic scanning imaging is completed. The device and the method have the advantages of being simple to adjust, high in zoom and axial tomographic speed and low in observation cost.

Description

technical field [0001] The invention relates to a microscopic imaging device and method, in particular to a zooming two-photon optical tweezers microscopic imaging device and method, which can realize separation and adjustment of a two-photon microscopic system with a common optical path, and complete three-dimensional two-photon scanning imaging, belonging to optical Microscopic imaging and optical manipulation technology. Background technique [0002] In conventional objective-type optical tweezers microscopes, the optical tweezers device and the imaging device are usually located on both sides of the sample, which is convenient for separate focusing. However, in some microscopic observation applications that require the introduction of other environmental variables (such as adding radiation The device is used to study the response mechanism of living cells to radiation), and the optical tweezers and the imaging device are required to be located on the same side of the sam...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/64G02B21/36
CPCG01N21/6458G02B21/361
Inventor 刘辰光刘俭谭久彬
Owner HARBIN INST OF TECH
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