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Method for extracting stevioside from stevia rebaudiana

A technology of steviol glycoside and stevia, which is applied in the field of stevioside, can solve the problems of cumbersome overall process, high impurity content, unfavorable application, etc., and achieve the effect of simple process, large specific surface area, and convenient concentration treatment

Inactive Publication Date: 2018-05-18
BENGBU HUADONG BIOLOGICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, the traditional extraction process of steviol glycosides in Stevia rebaudiana has high impurity content, many steps, and technical problems of cumbersome overall process, which are not conducive to application

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] A method for extracting steviol glycosides from Stevia rebaudiana, comprising the steps of:

[0025] S1. Grind 70kg of stevia dry leaves, add 550kg of water to soak for 30min, add 0.4kg of cellulase to hydrolyze for 30h, adjust the pH value of the system to 5.6-6.5, raise the temperature to 60°C for 4h, cool, settle and centrifuge, centrifuge speed For 3000r / min, filter to obtain the extract;

[0026] S2. Heat 240kg of the extract in a water bath, the temperature of the water bath is 90°C, add 1kg of flocculant and stir evenly, adjust the pH value of the system to 7.5-8.2, flocculate for 40min, centrifugally settle at a centrifugal speed of 12000r / min, filter, add 6kg of composite adsorbent was boiled for 4 hours under normal pressure reflux, filtered, and concentrated to obtain a concentrated solution;

[0027] The composite adsorbent is prepared by the following process: mix 25kg coconut shell carbon powder, 4kg vermiculite powder, 7kg sepiolite powder, 4kg medical s...

Embodiment 2

[0030] A method for extracting steviol glycosides from Stevia rebaudiana, comprising the steps of:

[0031] S1. Grind 90kg of stevia dry leaves, add 400kg of water to soak for 50min, add 0.2kg of cellulase to hydrolyze for 50h, adjust the pH value of the system to 5.6-6.5, raise the temperature to 50°C for 8h, cool, settle and centrifuge, centrifuge speed For 2500r / min, filter to obtain the extract;

[0032] S2. Heat 320kg of the extract in a water bath, the temperature of the water bath is 80°C, add 2kg of flocculant and stir evenly, adjust the pH value of the system to 7.5-8.2, flocculate for 20min, centrifugally settle at a centrifugal speed of 15000r / min, filter, add 4kg of composite adsorbent was boiled for 8 hours under normal pressure reflux, filtered, and concentrated to obtain a concentrated solution;

[0033] The composite adsorbent is prepared by the following process: mix 15kg coconut shell charcoal powder, 10kg vermiculite powder, 3kg sepiolite powder, 6kg medica...

Embodiment 3

[0036] A method for extracting steviol glycosides from Stevia rebaudiana, comprising the steps of:

[0037] S1. Crush 75kg of stevia dry leaves, add 500kg of water to soak for 35min, add 0.35kg of cellulase to hydrolyze for 32h, adjust the pH value of the system to 5.6-6.5, raise the temperature to 58°C for 5h, cool, settle and centrifuge, and the centrifuge speed For 2800r / min, filter to obtain the extract;

[0038] S2. Heating 280kg of the extract solution in a water bath, the temperature of the water bath is 88°C, adding 1.2kg of flocculant and stirring evenly, adjusting the pH value of the system to 7.5-8.2, flocculating for 35min, centrifuging and settling at a centrifugal speed of 13000r / min, filtering, Add 5.5 kg of composite adsorbent, cook for 5 hours under normal pressure reflux, filter, and concentrate to obtain a concentrated solution;

[0039] The composite adsorbent is prepared by the following process: mix 22kg coconut shell charcoal powder, 6kg vermiculite pow...

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PUM

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Abstract

The invention discloses a method for extracting stevioside from stevia rebaudiana. The method comprises the following steps: crushing dry leaves of stevia rebaudiana, adding water for soaking, addingcellulase for enzymolysis, and adjusting the pH value of the system to 5.6-6.5, and performing heating extraction, cooling, settling centrifugation and filtering to obtain an extract solution; heatingthe extract solution in a water bath, adding a flocculant, stirring the mixture uniformly, and adjusting the pH value of the system to 7.5-8.2, performing flocculating, centrifugally settling and filtering, adding a composite adsorbent, performing boiling in a normal pressure reflux state, and performing filtering and concentrating to obtain a concentrated solution; treating the concentrated solution on a silica gel chromatography column, performing eluting with a mixed solvent, collecting fractions, and performing vacuum concentrating and vacuum drying to obtain stevioside. The method has the advantages of simple process, low cost and high operability, and is suitable for factory production, the residual quantity of an organic solvent is quite low, obtained stevioside is white or off-white powder, and the purity and yield are high.

Description

technical field [0001] The invention relates to the technical field of steviol glycosides, in particular to a method for extracting steviosides from Stevia rebaudiana. Background technique [0002] Stevia rebaudiana, also known as stevia and sugar grass, belongs to Compositae. It contains 14 kinds of trace elements and 32 kinds of nutrients. It does not metabolize, accumulate, cause cancer and is non-toxic in the body. Its safety has been approved by the international FAO and WHO and the US FAD. organization, so it is both an excellent source of sugar and a good source of nutrition. [0003] Steviol glycoside is an active ingredient in stevia, which is a natural low-calorie high-intensity sweetener. Since stevioside has the above-mentioned high sweetness and low calorie characteristics, long-term consumption will not make people fat. Therefore, stevioside has been used as a sweetener to replace sucrose. To develop natural alternatives admired for their value and health. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H15/24C07H1/08
CPCC07H15/24C07H1/08
Inventor 邢飞邢伟张志华庞正银
Owner BENGBU HUADONG BIOLOGICAL TECH
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