Quick sperm dye liquor

Abstract

The invention discloses quick sperm dye liquor which is prepared from the following components in mass percent: 0.01% to 50% of brilliant cresyl blue, 0.01% to 50% of safranine O, 0.01% to 50% of newmethylene blue and the balance of water. The quick sperm dye liquor disclosed by the invention utilizes a one-step method to quickly dye ejaculated sperm cells and can dye different colors on the sperms and all kinds of cells in seminal fluid within 5 seconds to make sperm structures very clear, so that quick and accurate recognition can be obtained, reliable diagnosis basis is provided for clinicuse, important effect on accurately diagnosing and identifying male reproductive system diseases and precisely treating the male reproductive system diseases are achieved, and extremely important effects in seminal fluid dyeing standardization and quality control are achieved.

Description

technical field [0001] The invention relates to the technical field of sperm dyeing liquid products, in particular to a rapid sperm dyeing liquid. Background technique [0002] Sperm cell staining technology refers to the chemical staining of sperm in semen to display various microstructures inside the sperm, which is convenient for observing its shape and reflects the morphological characteristics under physiological and pathological conditions. Sperm cell morphology examination is of great value in the identification and diagnosis of clinical male reproductive system diseases, and semen cell staining technology is an important part of semen routine projects. [0003] Sperm morphology examination in semen routine items usually uses Wright-Geek staining method (see pages P123-124 of the third edition of the National Clinical Laboratory Operation Regulations) and Papanicolaou staining (see the third edition of the National Clinical Laboratory Operation Regulations P325-326 pa...

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Problems solved by technology

[0003] Sperm morphology examination in semen routine items usually uses Wright-Geek staining method (see pages P123-124 of the third edition of the National Clinical Laboratory Operation Regulations) and Papanicolaou staining (see the third edition of the National Clinical Laboratory Operation Regulations P325-326 pages ) and hematoxylin-eosin staining (see pages 326-327 of the third edition of the National Clinical Laboratory Operation Regulations), etc. These staining methods have many shortcomings such as complicated operation, long staining time, unstable color development, and toxic reagents.

[0004] Specifically, the main disadvantages of the Wright-Kie's staining method are: the result of sperm staining is unstable, easily affected by the pH of semen samples, slides and buffers, and the staining time is long (15 to 30 minutes). The coloring of the acrosome structure and tail structure of the sperm is not ideal; moreover, the Wright-Girl's stain contains flammable and toxic methanol, which poses a safety hazard

[0005] The main disadvantages of the Papanicolaou staining method are: the staining effect is good, but it is still not stable enough, and the staining process is very complicated, with many operation steps, including more than a dozen operation steps; the staining time is more than 1 hour, and the reagents Contains highly toxic metal mercury, polluting the environment

[0006] The main disadvantage of the hematoxylin-eosin staining method is that it is actually a simple Papanicolaou staining method. The staining effect is not as good as that of the Papanicolaou method, but the staining process is simpler than the Papanicolaou method. The operation steps include six steps; the staining time It takes nearly 1 hour, and the reagent contains highly toxic metal mercury, polluting the environment

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