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Degradation strain JN1 of petroleum hydrocarbons in oily sludge and application thereof

A technology of JN1 and degrading bacteria, applied in the field of bioremediation, can solve the problems of no large-scale application, cumbersome cultivation process operation, and great influence of environmental factors, so as to avoid complex reaction products, optimize soil microbial system, and enhance biological activity. Effect

Active Publication Date: 2018-05-15
PEKING UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Using metabolic surfactant microorganisms to treat oily sludge can increase the solubility of petroleum hydrocarbons, which can improve the degradation efficiency of petroleum hydrocarbons, and use biological treatment to remove harmful substances such as PAHs. Biological treatment can simultaneously degrade aliphatic hydrocarbons and aromatics in oily sludge However, the biological treatment method has limitations such as being greatly affected by environmental factors and a long treatment period, so the treatment technology for biological treatment of oily sludge has not yet been put into large-scale application in China
At the same time, the current microbial degradation bacterial agents are all composite microbial bacterial agents. Various bacterial strains need to be cultured and activated separately and then mixed in a certain proportion. cumbersome
In the prior art, there is no technical solution for using a single bacterial strain to achieve relatively excellent degradation of petroleum hydrocarbons in oily sludge

Method used

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  • Degradation strain JN1 of petroleum hydrocarbons in oily sludge and application thereof
  • Degradation strain JN1 of petroleum hydrocarbons in oily sludge and application thereof
  • Degradation strain JN1 of petroleum hydrocarbons in oily sludge and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] Example 1: Degradation and repair test of oily sludge in Liaohe Oilfield

[0056] Oily sludge from Liaohe Oilfield was taken, air-dried and ground, passed through a 1mm sieve, and set aside. Pick a single colony in sterilized LB liquid medium, seal it with a gas-permeable sealing film, place the Erlenmeyer flask in a shaker at 120 rpm, and activate at 30°C for 24 hours. Weigh 40g of the above-mentioned air-dried, ground and sieved oily sludge, add 10% inoculation amount of Acinetobacter sp.JN1 activated bacterial solution with OD600=1, adjust the water-to-soil ratio to 2:5 with inorganic salt medium, and seal it with air-permeable Membrane seal. All Erlenmeyer flasks were placed in a constant temperature incubator at 30°C for static cultivation, samples were taken every 5 days, and the content of total petroleum hydrocarbons in the oily sludge was measured with an infrared spectrophotometer. The degradation curve is shown in the attached image 3 shown. It can be see...

Embodiment 2

[0060] Example 2: Degradation effect of Acinetobacter sp.JN1 on petroleum hydrocarbons in oily sludge at different pH values ​​Oily sludge from Liaohe Oilfield was taken, air-dried, ground, and passed through a 1 mm sieve. Weigh 40 g of the above oily sludge in a conical flask, and use dilute H 2 SO 4 and NaOH to adjust the pH of the soil to be 1, 3, 5, 7, 9, 11 respectively, add the activated bacterial liquid of Acinetobacter sp.JN1 with OD600=1 according to the inoculation amount of 10%, and adjust the water-soil ratio to 2:5 with distilled water, Seal with breathable parafilm. All the Erlenmeyer flasks were placed in a constant temperature incubator at 30°C for static cultivation and samples were taken after 25 days, and the total petroleum hydrocarbon content in the oily sludge was determined by an infrared spectroscopic oil analyzer. The degradation effects of strain Acinetobacter sp.JN1 on petroleum hydrocarbons in oily sludge under different pH conditions are as follo...

Embodiment 3

[0061] Example 3: Degradation effect of Acinetobacter sp.JN1 on petroleum hydrocarbons in oily sludge at different temperatures

[0062] Oily sludge from Liaohe Oilfield was taken, air-dried, ground, and passed through a 1mm sieve. Weigh 40g of the above oily sludge into an Erlenmeyer flask, add 10% inoculation amount of Acinetobacter sp.JN1 activated bacterial solution with OD600=1, adjust the water-soil ratio to 2:5 with distilled water, and seal it with a breathable sealing film. The Erlenmeyer flasks were placed in constant temperature incubators at 10°C, 15°C, 20°C, 25°C, 30°C, 35°C, 40°C, 45°C, and 50°C for static culture, and samples were taken after 25 days. Spectrophotometer was used to determine the total petroleum hydrocarbon content in oily sludge. The degradation effect of strain Acinetobacter sp.JN1 on total petroleum hydrocarbons in oily sludge at different temperatures is as follows: Figure 5 shown. Depend on Figure 5 It can be seen that the strains of th...

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Abstract

The invention discloses an efficient degradation strain JN1 of petroleum hydrocarbons in oily sludge and application thereof, and belongs to the technical field of biological restoration. The degradation strain JN1 is preserved in CGMCC (China General Microbiological Culture Collection Center), has the preservation number of CGMCC NO.14972, and has the classification number of Acinetobacter sp.. The purpose of the strain JN1 with the petroleum hydrocarbon degradation function is the oily sludge control performed in a petroleum hydrocarbon degradation mode. The degradation rate of a single strain of the bacterium in 30 days on the TPH in the oily sludge is 58.05 percent; the most proper degradation conditions of the bacterium on petroleum contaminants are the follows: the temperature is 25to 50 DEG C and the pH value is 5 to 7. The biological method is used for the restoration technology of the oily sludge, and has the advantages of high efficiency, low cost, environment-friendly effect and the like.

Description

technical field [0001] The invention relates to the technical field of bioremediation, in particular to a bacterial strain for degrading and repairing oily sludge and its application in degrading oily sludge. Background technique [0002] Since the 20th century, petroleum has become one of the most important energy sources for human beings. While being exploited and used in large quantities, the harm caused by petroleum and its processed products to the environment and human beings has become increasingly serious. Since China's reform and opening up, my country's annual output of crude oil has exceeded 100 million tons. China has become one of the world's top ten oil-producing countries and has more than 400 oil fields and oil and gas fields for exploration and development, which are distributed in 25 countries accounting for about 3% of the country's land area. Provinces (municipalities) and autonomous regions. However, in the process of rapid economic and technological dev...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F11/02C12R1/01
CPCC02F11/02C12N1/205C12R2001/01
Inventor 叶正芳姜楠赵泉林
Owner PEKING UNIV
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