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Compound microbial fermentation agent and application thereof

A technology of compound microorganisms and starters, applied in the direction of microorganisms, microorganisms, methods based on microorganisms, etc., can solve the problems of complex and cumbersome preparation methods of compound microecological preparations, reduce the feed-to-egg ratio of laying hens, etc., achieve safe and stable feed quality, improve Egg production rate and effect of improving egg quality

Active Publication Date: 2018-05-15
TIANJIN BIOFEED TECH CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] CN103518980A discloses a preparation method and application of a microecological preparation for laying hens. The microecological preparation is mainly composed of Lactobacillus plantarum and Bacillus subtilis, but the invention does not reduce the feed-to-egg ratio of laying hens, and the composite microecological The preparation method of the preparation is complex and cumbersome. It needs to be cultured in a fermentation tank, mixed with an aqueous solution containing pectin and other components, and then spray-dried to make a microencapsulated live bacterial powder. Then the bacterial powder is mixed with selenized carrageenan and zinc methionine and auxiliary materials mixed evenly
CN102067941A discloses a production method of a compound microbial preparation for feed. Three strains of Bacillus subtilis, Saccharomyces cerevisiae, and Lactobacillus plantarum are selected and bred to form a compound strain, which can be combined with ball milled bran, several skins and isomaltooligosaccharides to form a good It can effectively improve the intestinal function of livestock and poultry, improve the feed conversion rate, and improve the micro-ecological balance of the intestinal tract of livestock and poultry. However, this invention only introduces the production method and has no application examples.

Method used

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  • Compound microbial fermentation agent and application thereof
  • Compound microbial fermentation agent and application thereof
  • Compound microbial fermentation agent and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] Prepare starter

[0059] Ferment Lactobacillus plantarum, Bacillus subtilis and Saccharomyces cerevisiae separately, and end the fermentation at the late logarithmic period, centrifuge the fermentation broth to collect wet bacteria, resuspend the bacteria with physiological saline or pH 6.8 phosphate buffer, press plant Lactobacillus: Bacillus subtilis: Saccharomyces cerevisiae is mixed in a ratio of 2:1:3 to obtain a composite cell, and the composite cell mixture is mixed with rice husk powder in a ratio of 1:5 to dry at low temperature to obtain a starter, wherein Lactobacillus plantarum The number of live bacteria ≥ 3.0×10 10 CFU / g, the number of live bacteria of Bacillus subtilis ≥2.0×10 9 CFU / g, the number of live bacteria of Saccharomyces cerevisiae ≥5.0×10 10 CFU / g.

[0060] Preparation of bio-fermented feed

[0061] (1) Weigh the starter and dissolve it in pure water at a ratio of 1:50, activate it at 35°C for 0.5h, and use it as a seed solution for later us...

Embodiment 2

[0071] Prepare starter

[0072] Ferment Lactobacillus plantarum, Bacillus subtilis and Saccharomyces cerevisiae separately, and end the fermentation at the late logarithmic period, centrifuge the fermentation broth to collect wet bacteria, resuspend the bacteria with physiological saline or pH 6.8 phosphate buffer, press plant Lactobacillus: Bacillus subtilis: Saccharomyces cerevisiae is mixed in a ratio of 4:3:7 to obtain a composite cell, and the composite cell mixture is mixed with rice husk powder in a ratio of 3:10 to dry at low temperature to obtain a starter, wherein Lactobacillus plantarum The number of live bacteria ≥ 3.0×10 10 CFU / g, the number of live bacteria of Bacillus subtilis ≥2.0×10 9 CFU / g, the number of live bacteria of Saccharomyces cerevisiae ≥5.0×10 10 CFU / g;

[0073] Preparation of bio-fermented feed

[0074] (1) Weigh the starter and dissolve it in pure water at a ratio of 1:50, activate it at 36°C for 1 hour and use it as seed liquid for later use...

Embodiment 3

[0080] Prepare starter

[0081] Ferment Lactobacillus plantarum, Bacillus subtilis and Saccharomyces cerevisiae separately, and end the fermentation at the late logarithmic period, centrifuge the fermentation broth to collect wet bacteria, resuspend the bacteria with physiological saline or pH 6.8 phosphate buffer, press plant Lactobacillus: Bacillus subtilis: Saccharomyces cerevisiae is mixed in a ratio of 2:3:3, and the mixture of complex bacteria and stone powder is mixed in a ratio of 2:7 to obtain a starter at low temperature, wherein the number of viable Lactobacillus plantarum is ≥3.0×10 10 CFU / g, the number of live bacteria of Bacillus subtilis ≥2.0×10 9 CFU / g, the viable count of Saccharomyces cerevisiae ≥5.0×10 10 CFU / g;

[0082] Preparation of bio-fermented feed

[0083] (1) Weigh the starter and dissolve it in pure water at a ratio of 1:50, activate it at 34°C for 0.8h, and use it as a seed solution for later use;

[0084] (2) take raw material by formula quant...

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Abstract

The invention provides a compound microbial fermentation agent and application thereof. The fermentation agent is prepared from composite thalli and carriers according to a mass ratio of (1 to 3):(5 to 10). The composite thalli comprise lactobacillus plantarum, bacillus subtilis and saccharomyces cerevisiae. Active strains are scientifically screened; the strains and the specific carriers are mixed; the strain proportioning ratio and the carrier addition quantity are optimized to obtain the novel microbial fermentation agent; a proper amount of the microbial fermentation agent is added into feed raw materials; the fermentation technical flow process is regulated; finally, the novel biological fermentation feed with the safe and stable quality and balanced nutrition can be prepared; after the feed is fed to laying hens, the production performance of the laying hens can be effectively improved; the quality of egg products can be improved; the culture cost is reduced; the economic benefits are obviously improved.

Description

technical field [0001] The invention relates to the technical field of biological fermentation, and mainly relates to a composite microbial fermentation agent and its application. Background technique [0002] For a long time, eggs, as a cheap and popular animal food, are an important source of protein for human beings, and are deeply loved by consumers at home and abroad. At present, although the eggs produced by high-density large-scale breeding hens have high yield and large individual volume, their nutritional value is often not as good as that produced by ordinary farm hens. Egg ratio and feed-egg ratio are also important factors affecting the production efficiency of chicken farms. Only by improving the comprehensive production performance of laying hens can the maximum benefit be realized. Thereby how to improve the productive performance of laying hen, improve egg quality, be the major difficult problem that research and development personnel face always. [0003] ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/16A23K10/12A23K10/30A23K10/37A23K20/147A23K10/33A23K20/20A23K50/75C12R1/25C12R1/125C12R1/865
CPCA23K10/12A23K10/30A23K10/33A23K10/37A23K20/147A23K20/20A23K50/75C12N1/16C12N1/20C12R2001/125C12N1/205C12R2001/25C12R2001/865C12N1/185A23V2400/169Y02P60/87
Inventor 邓雪娟王春雨刘世杰田子罡陈达蔡辉益程善燕
Owner TIANJIN BIOFEED TECH CO LTD
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