Preparation method of adenylosuccinic acid or adenylosuccinate

Abstract

The invention belongs to the technical field of scale production of candidate medicines for treating glucose-lipid metabolism disorder diseases. In order to solve the problems that existing S-AMP is difficultly produced, the price of the S-AMP is expensive, and raw material sources of the druggability of the S-AMP is difficulty ensured, the invention provides a preparation method of adenylosuccinic acid or adenylosuccinate. PhAdSS of which the N terminal is provided with an His-tag sequence is expressed by utilizing a pET-28-a carrier; with the PhAdSS as a catalyst, inosinic acid IMP, L-aspartic acid and guanosine triphosphate (GTP) are catalyzed and subjected to reaction to synthesize S-AMP; after the reaction is carried out, components in a product are analyzed by adopting silica gel thin-layer chromatography, and separation and purification on the S-AMP are carried out by adopting silica gel coluumn chromatography. The content of the s-AMP in a synthetic product is 61 percent, and arecycling ratio is about 20 percent. A preparation process is simple, the cost is low, and a basis is laid for building an industrialized S-AMP synthetic process; monopoly sale on S-AMP by foreign sellers is expected to be broken through, and the S-AMP druggability research cost can be greatly reduced.

Description

technical field [0001] The invention belongs to the technical field of large-scale production of candidate drugs for the treatment of disorders of glucose and lipid metabolism, and in particular relates to a preparation method of adenylosuccinic acid or a salt thereof and an application thereof. Using enzymes from deep-sea archaea to catalyze the synthesis of a natural adenosine compound: adenylyl succinic acid (salt), a small-scale laboratory preparation method was established. This compound has the function of promoting insulin secretion, and can be used as a candidate drug for improving glucose and lipid metabolism disorders to carry out druggability research. Background technique [0002] Adenylosuccinic acid (Adenylosuccinate), referred to as S-AMP] is a natural adenosine compound that promotes insulin secretion and restores the normal function of islet cells in patients with type II diabetes [1]. In previous studies, we found that S-AMP is an agonist of AMPK, and its ...

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Problems solved by technology

[0004] In order to solve the problem that the existing adenylyl succinic acid, that is, S-AMP, is difficult to produce, its price is expensive, and it is difficult to ensure the source of raw materials for its druggability research, the present invention provides a preparation method of adenylyl succinic acid or a salt, It is a small-scale synthesis and purification technology of S-AMP using enzymatic reaction, and a small-scale synthesis system of S-AMP was established by using PhAdSS, which laid the foundation for the establishment of the industrial production process of S-AMP

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