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Quick detection method of glucocorticoid in cosmetics and application

A technology of glucocorticoid and detection method, which is applied in the direction of analysis by chemical reaction of materials and material analysis by observing the influence on chemical indicators, to achieve the effects of high accuracy, strong specificity and wide application range

Inactive Publication Date: 2018-05-08
WENZHOU VOCATIONAL COLLEGE OF SCI & TECH
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  • Claims
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Problems solved by technology

For consumers, the relatively expensive testing fee and cumbersome process are unacceptable for sending to institutions for testing, so rapid testing is very necessary to deter illegal manufacturers, administrative law enforcement, and consumer self-inspection

Method used

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  • Quick detection method of glucocorticoid in cosmetics and application

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Experimental program
Comparison scheme
Effect test

preparation example Construction

[0026] 1) Preparation of samples: Take 1-2g of cosmetics, add 2-10mL of methanol to extract, stir and mix well, and shake by hand; directly take 1-2mL of liquid samples and add 2mL of methanol. Take the upper layer liquid, supernatant or filtrate as the extraction liquid;

[0027] 2) Detection reagent configuration: nitro blue tetrazolium chloride is dissolved in methanol solution of sodium hydroxide with a solvent of 50-400g / kg, and nitro blue tetrazolium chloride methanol solution is 0.1-2g / L, as a detection Reagent;

[0028] 3) Detection: In the extract solution, add 2 drops of strong alkali solution of methanol dissolved in nitroblue tetrazolium chloride, and observe the color change; the color of the solution is blue or blue-green, and the color change of different glucocorticoids is slightly There is a difference.

[0029] The purpose of the present invention is to provide the above-mentioned method in checking whether to add glucocorticoids in cosmetics

[0030] Appl...

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Abstract

The invention discloses a quick detection method of glucocorticoid in cosmetics and application. The quick detection method comprises the steps of sample extraction, detection reagent preparation anddetection. According to the method, whether a sample contains the glucocorticoid can be detected quickly and semiquantitatively when a strong-base methanol solution of nitro blue tetrazolium chloridemonohydrate specifically reacts with a functional group of the glucocorticoid to generate a color; high cost and large equipment are not required; and the method is quick in detection, cheap, convenient, quick and safe.

Description

technical field [0001] The invention belongs to the technical field of cosmetic detection and detection, and more specifically relates to a rapid detection method for cosmetic glucocorticoids, and at the same time, the invention also relates to the application of a rapid detection method for cosmetic glucocorticoids. Background technique [0002] Adding sex hormones to cosmetics can quickly achieve hair growth, breast enhancement, breast beautification, whitening and other functions, but long-term use will increase the incidence of breast cancer and uterine fibroids in women, and also cause irregular menstruation, pigmentation, and skin thinning and other adverse reactions. Glucocorticoids can regulate the biosynthesis and metabolism of sugar, fat, and protein. Short-term use can make the skin smooth and delicate, white and rosy, and play a cosmetic role, but long-term use can lead to endocrine system disorders and lead to A series of side effects, such as high blood pressu...

Claims

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Application Information

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IPC IPC(8): G01N21/78
CPCG01N21/78
Inventor 王亮
Owner WENZHOU VOCATIONAL COLLEGE OF SCI & TECH
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