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A kind of camellia oleifera polysaccharide, preparation method and the effect of antagonizing foot-and-mouth disease vaccine on bull semen damage

A kind of foot-and-mouth disease vaccine, camellia meal technology

Active Publication Date: 2019-05-03
安庆旭康农业发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, there is no report on the in-depth development of polysaccharides from tea leaf

Method used

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  • A kind of camellia oleifera polysaccharide, preparation method and the effect of antagonizing foot-and-mouth disease vaccine on bull semen damage
  • A kind of camellia oleifera polysaccharide, preparation method and the effect of antagonizing foot-and-mouth disease vaccine on bull semen damage
  • A kind of camellia oleifera polysaccharide, preparation method and the effect of antagonizing foot-and-mouth disease vaccine on bull semen damage

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1: Preparation and average molecular weight determination of camellia oleifera polysaccharide 1-3

[0031] Including the following steps:

[0032] Step S1, crush the small fruit oil tea meal to 80-100 mesh, take 1 kg and add 15 L of boiling water to reflux extraction for 3 times, each extraction for 2 hours, filter each extraction, combine the filtrates, concentrate under reduced pressure to 150 mL, and use the Sevag method deproteinized;

[0033] Step S2, add 4 times the volume of absolute ethanol to the concentrated solution after deproteinization, stir evenly and place it at 4°C for 24 hours at low temperature, 5×10 3 Centrifuge at rpm for 5 minutes to collect the precipitate;

[0034] Step S3, dissolve the precipitate in deionized water, add the sample to the DEAE-52 chromatographic column, and sequentially elute with pure water, 0.3mol / L sodium chloride solution, and 0.5mol / L sodium chloride solution for 15 Column volume, flow rate 1.0mL / min, collect pur...

Embodiment 2

[0040] Example 2: Camellia oleifera meal polysaccharide 1-3 antagonizes bull semen damage caused by foot-and-mouth disease vaccine

[0041] 1. Experimental materials

[0042] The bull breed is Holstein; the bull semen diluent uses TCM-199.

[0043] The foot-and-mouth disease vaccine is a bivalent inactivated vaccine of foot-and-mouth disease virus type O Asia type I, and the inoculation dose is 2.5mL / head.

[0044] Accurately weigh polysaccharides 1-3 of camellia oleifera meal and dissolve them in physiological saline to prepare an injection solution with a concentration of 5 mg / mL.

[0045] 2. Experimental method

[0046] 1. Collection, inspection, and grouping of semen (reference for collection and inspection methods: Research on the Effect of Foot-and-Mouth Disease Vaccine Injection on the Semen Quality of Breeding Bulls, China Cattle Industry Science, 2014)

[0047]The instruments used should be rinsed and sterilized in advance; the glassware should be washed and sent t...

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Abstract

The invention discloses camellia oleifera meal polysaccharide, a preparation method and an effect of a foot-and-mouth disease antagonizing vaccine on bull semen damage. The camellia oleifera meal polysaccharide is separated from camellia meiocarpa, and the average molecular weight is 75-95 kDa. The preparation method includes the specific steps that S1, the camellia meiocarpa is crushed, extracted, filtered and concentrated; S2, concentrated solution is added with anhydrous ethanol, and precipitates are collected after centrifugation; S3, the precipitates are dissolved in deionized water, samples are added in a DEAE-52 column, pure water and sodium chloride solution with different concentrations are successively used for gradient elution, and pure water eluent and 0.3mol / L, 0.5mol / L sodiumchloride eluent are collected respectively; S4, the pure water eluent and the 0.3mol, 0.5 mol / L sodium chloride eluent are concentrated on a Sephadex G-100 column, deionized water is used for elution, elution peaks are collected respectively, and freeze drying is conducted. The camellia oleifera meal polysaccharide can effectively antagonize damage on bull semen caused by the foot-and-mouth disease vaccine.

Description

technical field [0001] The invention belongs to the field of animal husbandry, and relates to a camellia oleifera polysaccharide, a preparation method and antagonizing the effect of a foot-and-mouth disease vaccine on bull semen damage. Background technique [0002] Camellia meiocarpa Hu. is the main species of Camellia oleifera (Camellia oleifera Abel.) in terms of cultivation area and annual output in my country. It is mainly distributed in Fujian, Jiangxi and Guangxi south of Huaihe River and Yangtze River in my country. , Hunan, Guizhou and other places. Small fruit camellia is also known as Jiangxizi, small tea, chicken heart, small leaf camellia, sheep excrement and so on. The fruit is small, the leaves are small, the buds are small, and the buds and bracts are not hairy. [0003] Camellia oleifera meal is a by-product obtained after extracting oil from Camellia oleifera. Although tea farms have detoxified these camellia leaves and used them as animal feed, due to th...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/715C08B37/00A61P39/00
CPCC08B37/0003C08B37/0006
Inventor 盛蓓蓓李沛王崇智
Owner 安庆旭康农业发展有限公司
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