Mariculture fish multiple anti-idiotypic egg yolk antibody vaccine and preparation method thereof

An anti-idiotype, yolk antibody technology, applied in the direction of multivalent vaccines, antibodies, vaccines, etc., can solve the problems of reducing the growth activity of pathogens, unable to eliminate internal and external toxins of pathogens, toxic and side effects, etc., to achieve aseptic processing and easy access to materials. , the effect of enhancing resistance

Active Publication Date: 2018-05-08
西安德轩驰生物科技有限公司
View PDF10 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The main disadvantage of this vaccine is that the inactivation or attenuation method can only eliminate or reduce the growth activity of the pathogen, but cannot eliminate the internal and external toxins contained in the pathogen itself, and improper handling can easily produce toxic side effects

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Mariculture fish multiple anti-idiotypic egg yolk antibody vaccine and preparation method thereof
  • Mariculture fish multiple anti-idiotypic egg yolk antibody vaccine and preparation method thereof
  • Mariculture fish multiple anti-idiotypic egg yolk antibody vaccine and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] 1. Preparation of marine fish disease immunogen

[0030] According to the investigation and research report of pathogenic bacteria in the coastal waters of my country, the main pathogenic bacteria along the coast of my country are Vibrio anguillarum, Edwardsiella, Vibrio alginolyticus, Vibrio parahaemolyticus, Vibrio vulnificus, and Streptococcus. The standard strains of the above-mentioned marine fish pathogens were purchased from international and domestic cell banks, amplified, cultured, and tested to determine the 50% lethal concentration of each pathogen. The half lethal dose of the six pathogens is 1×10 4 ~1×10 5 / ml.

[0031] 2. Preparation of marine fish disease antibodies

[0032] Immunization: The half-lethal concentration of various pathogenic bacteria is fixed and inactivated by formaldehyde fixative solution, and the volume of Freund's incomplete adjuvant is mixed 1:1 to immunize mice and rabbits respectively. Each mouse is injected intraperitoneally to immunize ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention provides a preparation method of a mariculture fish multiple anti-idiotypic egg yolk antibody vaccine. The preparation method comprises the following steps of after inactivating mariculture fish pathogenic bacteria, immunizing mice and rabbits; after using six rabbit anti-pathogenic serums to be mixed with the inactivated mariculture fish pathogenic bacteria, immunizing laying hens;and screening the laying hens, collecting, refining and purifying an obtained coarse anti-idiotypic egg yolk antibody and the mariculture fish multiple anti-idiotypic egg yolk antibody vaccine, wherein the mariculture fish pathogenic bacteria are vibrio anguillarum, Edwardsiella tarda, vibrio alginolyticus, vibrio parahaemolyticus, vibrio vulnificus and streptococcus. The multiple anti-idiotypic egg yolk antibody vaccine provided by the invention can strengthen the resistance of marine fishes to various fish diseases, can prevent various marine fish diseases, can also perform immunization to the marine fishes through an oral way, is simple, convenient and feasible and is liable in obtaining materials at the aspect of preparing an anti-idiotypic egg yolk antibody, is convenient with aseptictreatment, is simple and convenient in preparation programs, low in cost and high in yield and accords with national policy orientation of energy conservation, environmental protection and sustainable development.

Description

Technical field [0001] The invention belongs to the field of aquatic vaccines, and in particular relates to a polyconjugate anti-idiotype egg yolk antibody vaccine for marine cultured fish and a preparation method thereof. Background technique [0002] my country is the country with the largest production of mariculture fish, with 7 billion mariculture fish each year. As the breeding density increases, the water environment continues to deteriorate, and the diseases are becoming more and more serious. The mariculture animals that die from diseases every year cause more than 10 billion economic losses to the country. The main diseases that harm our mariculture animals are bacterial diseases and viral diseases. Bacterial diseases such as Vibrio anguilla, Edwards, Vibrio parahaemolyticus, Vibrio vulnificus, Streptococcus, Vibrio alginolyticus; Viral diseases such as iris virus, shrimp white spot virus and nervous necrosis virus, etc., they cause a large number of marine animals Th...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C07K16/42C07K16/02A61K39/395A61P31/04
CPCA61K2039/507A61K2039/552A61K2039/70C07K16/02C07K16/4233C07K2317/11C07K2317/35
Inventor 黄威权赵锋李丹
Owner 西安德轩驰生物科技有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap