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Chicken ring RNAChr26:2670958/2679178 detection primer, method and application

A detection method and a technology for detecting primers, which are applied in the field of genetic engineering and can solve problems such as drug resistance, food safety bacteria, and enhanced drug resistance of bacterial strains

Inactive Publication Date: 2018-05-04
SHANDONG AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, the main method to control Salmonella infection is the use of vaccines and antibiotics, but this cannot fundamentally solve the problem, and the extensive use of antibiotics will cause food safety problems and bacterial drug resistance, and lead to enhanced drug resistance of bacterial strains

Method used

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  • Chicken ring RNAChr26:2670958/2679178 detection primer, method and application
  • Chicken ring RNAChr26:2670958/2679178 detection primer, method and application
  • Chicken ring RNAChr26:2670958/2679178 detection primer, method and application

Examples

Experimental program
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Embodiment 1

[0032] Example 1 Establishment of the detection method for RNA Chr26:2670958|2679178

[0033] Select individuals negative for Salmonella Enteritidis, and inoculate Salmonella Enteritidis by oral method, the inoculation volume is 10 7 -10 9 cfu / only, on the 7th day after inoculation, the contents of the cecum were collected, and the bacterial content was determined by plate counting method. Take cecal tissue samples, extract RNA, and process the extracted RNA with RNase R to digest linear RNA, and perform reverse transcription to generate cDNA.

[0034] According to the gene sequence of chicken circular RNA Chr26:2670958|2679178, specific circular and linear primers (Table 1 and Table 2) were designed for amplification. Artificial sequence

[0035] Table 1: Chicken circular RNA Chr26:2670958|2679178 circular primer sequence

[0036]

[0037] Table 2: Chicken circular RNA Chr26:2670958|2679178 linear primer sequence

[0038]

[0039] Quantitative PCR amplification was...

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Abstract

The invention discloses a chicken ring RNA Chr26:2670958 / 2679178 detection primer, a method and application. The primer is prepared from a chicken ring RNA Chr26: 2670958 / 2679178 annular upstream primer body and a chicken ring RNA Chr26: 2670958 / 2679178 annular downstream primer body, wherein nucleotide sequences of the chicken ring RNA Chr26: 2670958 / 2679178 annular upstream primer body and the chicken ring RNA Chr26: 2670958 / 2679178 annular downstream primer body are respectively shown in SEQ ID NO.1 and SEQ ID NO.2. By means of the method disclosed by the invention, the relative expressionsituation of the chicken ring RNA Chr26: 2670958 / 2679178 can be accurately detected after a chicken is infected by salmonella enteritidis; the relative expression situation is utilized as a molecularmarker to identify salmonella infection resistance individuals; thus, theoretical basis and scientific basis are provided for salmonella enteritidis infection resisting genetic breeding of chickens.

Description

technical field [0001] The invention belongs to the technical field of genetic engineering, and in particular relates to a detection primer, method and application of chicken circular RNAChr26:2670958|2679178. Background technique [0002] Salmonella enteritidis (SE) is a common zoonotic pathogen. Because it has no host specificity, it can not only cause diseases such as acute gastroenteritis in poultry and even cause death, causing huge economic losses to the poultry industry, but also seriously endangering humans. Health, causing food poisoning, is one of the important pathogenic bacteria that affect people's physical and mental health. Relevant reports pointed out that during 2004, 19,000 people per 1 million people in the United States were hospitalized due to Salmonella infection, and as many as 300 people died due to Salmonella infection; Disease outbreak. Poultry and related processed products are the main source of infection of Salmonella Enteritidis, and the cecum...

Claims

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Application Information

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IPC IPC(8): C12Q1/689C12Q1/10C12N15/11
CPCC12Q1/689C12Q2600/158C12Q2600/178Y02A50/30
Inventor 李显耀郑林娜刘丽英王园美
Owner SHANDONG AGRICULTURAL UNIVERSITY
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