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Primer pair, primer group, kit and method for distinguishing yak and cattle

A primer pair and primer set technology, applied in the field of primer pairs for distinguishing yak and cattle, can solve problems such as infringement of consumer rights, disrupting market order, and impact on the healthy development of the yak industry.

Active Publication Date: 2018-05-04
新羿制造科技(河北)有限公司
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

It not only disrupts the normal market order and violates the rights and interests of consumers, but also affects the healthy development of the yak industry

Method used

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  • Primer pair, primer group, kit and method for distinguishing yak and cattle
  • Primer pair, primer group, kit and method for distinguishing yak and cattle
  • Primer pair, primer group, kit and method for distinguishing yak and cattle

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Experimental program
Comparison scheme
Effect test

Embodiment

[0052] 1. Sample collection and genomic DNA extraction

[0053]56 samples of yak meat and 53 samples of local yellow beef were collected, including 16 samples of red beef from Jiaxian County, 15 samples of yellow beef from Nanyang, and 22 samples of yellow beef from Luxi. A total of 109 beef samples were collected. Genomic DNA was extracted separately. DNA was extracted using Thermo#k0512 DNA Extraction Kit. Specific steps are as follows:

[0054] (1) Take 30 mg tissue sample, put it in a 1.5 ml centrifuge tube, and add 200 μl TE. Add 400 μl of lysis solution to 200 μl of tissue samples, and incubate at 65° C. for 5 minutes. (Frozen samples: add 400μl lysissolution before thawing, incubate at 65°C for 10min.)

[0055] (2) Immediately add 600 μl of chloroform, invert 3-5 times to emulsify, and centrifuge at 10,000 rpm for 2 min.

[0056] (3) Preparation of precipitation reagent: 720 μl ddH 2 0 + 80 μl (10×) Precipilation Solution.

[0057] (4) Transfer the upper aqueous ...

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Abstract

The invention relates to the technical field of molecular identification, in particular to a primer pair, a primer group, a kit and a method for distinguishing yak and cattle. The primers can be usedfor amplifying a 150bp differential fragment of the yak and the cattle. The identification method provided by the invention comprises the major steps of sample genome DNA extraction, PCR amplificationand sample identification. The identification method has the characteristics of high speed, simplicity, economical performance and accuracy; the application to large-scale detection of fresh meat, commercial jerky or bone powder product samples can be realized. The method building achieves the important significance in aspects of yak food inspection and quarantine, commercial fraud prevention andfood backtracking investigation system building.

Description

technical field [0001] The invention relates to the technical field of molecular identification, in particular to a pair of primers, a primer set, a kit and a method for distinguishing yaks and cattle. Background technique [0002] In recent years, yak meat has been increasingly favored by consumers due to its high protein, low fat and no pollution. Due to the large difference in its price compared with other common beef, it is often impersonated by other low-priced yellow beef in the meat market. It not only disrupts the normal market order and violates the rights and interests of consumers, but also affects the healthy development of the yak industry. Therefore it is urgent to develop an effective method to identify the source of beef on the market. The DNA molecular detection technology with high sensitivity can identify the authenticity of beef from different sources. This method is convenient and simple to operate, and can efficiently and accurately identify the authe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888
Inventor 陈爱亮赵杰杨曙明李婷婷
Owner 新羿制造科技(河北)有限公司
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