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Primer pair, primer set, kit and method for distinguishing yaks from cattle

A primer pair and primer set technology, which is applied in the field of primer pairs for distinguishing yaks from cattle, can solve problems such as infringement of consumer rights, disruption of market order, and impact on the healthy development of the yak industry

Active Publication Date: 2020-08-04
新羿制造科技(河北)有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

It not only disrupts the normal market order and violates the rights and interests of consumers, but also affects the healthy development of the yak industry

Method used

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  • Primer pair, primer set, kit and method for distinguishing yaks from cattle
  • Primer pair, primer set, kit and method for distinguishing yaks from cattle
  • Primer pair, primer set, kit and method for distinguishing yaks from cattle

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Experimental program
Comparison scheme
Effect test

Embodiment

[0052] 1. Sample collection and genomic DNA extraction

[0053]56 yak beef samples and 53 local yellow beef samples were collected, including 16 Jiaxian red beef samples, 15 Nanyang yellow beef samples, and 22 Luxi yellow beef samples, for a total of 109 beef samples. Genomic DNA was extracted separately. DNA was extracted using Thermo#k0512 DNA extraction kit. Specific steps are as follows:

[0054] (1) Take 30 mg of tissue samples, put them in a 1.5 ml centrifuge tube, and add 200 μl of TE. 400 μl of lysis solution was added to 200 μl of tissue samples, and incubated at 65° C. for 5 min. (Frozen samples: add 400μl lysissolution before thawing, invert, incubate at 65°C for 10min.)

[0055] (2) Immediately add 600 μl of chloroform, invert 3-5 times to emulsify, and centrifuge at 10,000 rpm for 2 min.

[0056] (3) Preparation of precipitation reagent: 720 μl ddH 2 O + 80 μl (10×) Precipilation Solution.

[0057] (4) Transfer the DNA-containing upper aqueous phase to a ne...

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Abstract

The invention relates to the technical field of molecular identification, in particular to a primer pair, a primer group, a kit and a method for distinguishing yak and cattle. The primers can be usedfor amplifying a 150bp differential fragment of the yak and the cattle. The identification method provided by the invention comprises the major steps of sample genome DNA extraction, PCR amplificationand sample identification. The identification method has the characteristics of high speed, simplicity, economical performance and accuracy; the application to large-scale detection of fresh meat, commercial jerky or bone powder product samples can be realized. The method building achieves the important significance in aspects of yak food inspection and quarantine, commercial fraud prevention andfood backtracking investigation system building.

Description

technical field [0001] The invention relates to the technical field of molecular identification, in particular to a primer pair, primer set, kit and method for distinguishing yak and cattle. Background technique [0002] In recent years, yak meat has been increasingly favored by consumers because of its high protein, low fat, and no pollution. Because its price is quite different from other common beef, it is often impersonated by other low-priced yellow beef in the meat market. It not only disrupts the normal market order and violates the rights and interests of consumers, but also affects the healthy development of the yak industry. Therefore, there is an urgent need to develop an effective method to identify the source of beef in the market. High-sensitivity DNA molecular detection technology can identify the authenticity of beef from different breeds. This method is convenient and simple to operate, and can efficiently and accurately identify the authenticity of yak an...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888
Inventor 陈爱亮赵杰杨曙明李婷婷
Owner 新羿制造科技(河北)有限公司
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