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Probes, systems, cartridges, and methods of use thereof

A technology of probes and porous materials, applied in probes, systems, boxes and their fields of use, can solve problems affecting probe performance, incompatibility, etc.

Inactive Publication Date: 2018-04-24
PURDUE RES FOUND INC +3
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Also, the sharp tip of the paper nebulizer probe directly affects the performance of the probe, and mass production processes such as die-cutting used to manufacture paper substrates have incompatibility issues with sharp tips made of paper

Method used

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  • Probes, systems, cartridges, and methods of use thereof
  • Probes, systems, cartridges, and methods of use thereof
  • Probes, systems, cartridges, and methods of use thereof

Examples

Experimental program
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Effect test

example 1

[0060] Example 1: Method

[0061] All chemicals were purchased from Sigma-Aldrich (St. Louis, MO, USA). Bovine whole blood was purchased from Innovative Research (Novi, MI, USA). Chromatographic papers (grade 1 and ET31 ) used to make paper substrates were purchased from Whitman (Whatman International Ltd, Maidstone, UK). Fused silica catheters (O.D. 130 μm, I.D. 50 μm) for paper capillary nebulizers were purchased from Molex Inc. (Lisle, IL, USA). Using a QTrap 4000 mass spectrometer equipped with an atmospheric pressure interface (API) (Applied Biosystems, Toronto, CA), using curtain gas and a domestic micro mass spectrometer, a miniature mass spectrometer with a discontinuous atmospheric pressure interface 12 Perform MS analysis.

[0062]For paper sprayers, spray substrates were prepared by cutting paper into triangles with a base of 6 mm and a height of 10 mm. An alligator clipper was used to hold the paper substrate during paper spraying with a 3.5 kV DC voltage app...

example 2

[0063] Example 2: Sample Analysis Using Probes of the Invention

[0064] Figure 6 Comic picture A shows the system of the present invention. The system includes a probe that includes a porous material and a hollow member (eg, a hollow capillary). A probe is coupled to the electrode via a porous material, and the probe generates ions that are expelled from the hollow member to a mass spectrometer, such as a micro mass spectrometer. The paper-capillary device of the present invention can be fabricated in two different ways. Paper substrates can be split on one side with a razor blade to facilitate capillary insertion ( Figure 6 comic strip B); or a cut can be made halfway through the ET31 paper substrate, and then the capillary can be pushed and embedded into the cut ( Figure 6 Comic picture C). No significant performance differences were observed between devices made by these two methods. However, the latter approach may be more suitable for mass production of device...

example 3

[0070] Example 3: Analysis of compounds using probes of the invention

[0071] now refer to image 3 , which shows using something like figure 1 Analysis of 50 ng / mL cocaine in bovine blood by the apparatus in Panel B and a TSQ mass spectrometer (Thermo Scientific, San Jose, CA). Whitman 31ET paper with a thickness of 0.4 mm was used to make the trapezoidal shaped substrate. An 8 mm fused silica capillary (49 μm i.d. and 150 μm o.d.) was inserted into the substrate at a depth of approximately 3 mm. A 5 μL blood sample was loaded onto a paper substrate to form a dried blood spot. 30 μL of methanol was applied to the substrate to facilitate analyte extraction and spray ionization. Apply 3000V to initiate the spray. a) Chronogram of extracted ions recorded using SRM transition m / z 304 to 182. b) MS / MS spectrum of the precursor m / z 304.

[0072] now refer to Figure 4 , which shows using something like figure 1 Analysis of 10 ng / mL cocaine and 30 ng / mL verapamil in me...

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Abstract

The invention generally relates to probes, systems, cartridges, and methods of use thereof. In certain embodiments, the invention provides a probe including a porous material and a hollow member coupled to a distal portion of the porous material. The invention provides probes that interface well with mass spectrometers that employ a curtain gas and with miniature mass spectrometers. Aspects of theinvention are accomplished by adding a hollow member (e.g., capillary emitter) to a porous substrate (e.g., paper substrate) for a paper-capillary spray. The data herein show that probes of the invention had significant, positive impact on the sensitivity and reproducibility for direct mass spectrometry analysis. The paper-capillary devices were fabricated and characterized for the effects due tothe geometry, the treatment to the capillary emitters, as well as the sample disposition methods.

Description

[0001] related application [0002] This application claims the benefit and priority of U.S. Provisional Application Serial No. 62 / 112,799, filed February 6, 2015, and U.S. Provisional Application Serial No. 62 / 211,268, filed August 28, 2015, each of which The contents are incorporated herein by reference in their entirety. [0003] governmental support [0004] This invention was made with government support under GM106016 awarded by the National Institutes of Health. The government has certain rights in this invention. technical field [0005] The present invention generally relates to probes, systems, cartridges and methods of use thereof. Background technique [0006] Paper nebulizers have been developed for direct mass spectrometry analysis of composite samples. This direct mass spectrometry has been implemented for sample analysis on commercial lab scale mass spectrometers as well as micro mass spectrometers. Since its development, a unique set of advantages of...

Claims

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Application Information

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IPC IPC(8): H01J49/04
CPCH01J49/0409H01J49/0445H01J49/26H01J49/167
Inventor 欧阳证任跃王骁
Owner PURDUE RES FOUND INC
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