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Nerve conduit with silk fibroin nanofiber directional guiding function and preparation method of nerve conduit

A silk fibroin and nanofiber technology, applied in the field of nerve conduits, can solve the problem of not having three-dimensional micro-nano guiding signals, and achieve the effect of a simple process.

Active Publication Date: 2018-04-24
WUHAN TEXTILE UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The invention patent with the authorized publication number CN100382772C uses silk fiber and silk fibroin to prepare nerve conduits, but there is no three-dimensional micro-nano guidance signal for directional guidance inside the conduit

Method used

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  • Nerve conduit with silk fibroin nanofiber directional guiding function and preparation method of nerve conduit
  • Nerve conduit with silk fibroin nanofiber directional guiding function and preparation method of nerve conduit

Examples

Experimental program
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Effect test

Embodiment 1

[0027] Soak 100g silkworm raw silk in 5L of 0.05% Na 2 CO 3 In the solution, boil at 98-100°C for 30min, repeat 3 times to degumming the silk, wash and dry it thoroughly to obtain pure silk fiber; add the silk fiber to 9.3mol / L LiBr solution, at 60°C Stir and dissolve for 1 hour to obtain a silk fibroin mixed solution; put the obtained silk fibroin mixed solution into a dialysis bag, and dialyze with deionized water for 3 days to obtain a purified silk fibroin solution;

[0028] Adjust the mass concentration of silk fibroin solution to 5%, pour it into the nerve guide mold, freeze at -80°C for 24h to form a frozen solid silk fibroin, then place it at -4°C for 3 days to induce the structural transformation of silk fibroin, and then Freeze-drying was carried out to obtain the outer layer catheter.

[0029] According to the method disclosed in patent CN106421902A, a rapidly gelled silk fibroin solution is prepared.

[0030] The solution was diluted to 0.2%, poured into the abo...

Embodiment 2

[0036] The 2% chitosan solution was poured into the nerve conduit mold, frozen at -80° C. for 24 hours, and then freeze-dried to obtain the outer layer chitosan conduit.

[0037] According to the method disclosed in the patent CN101502670B, a rapidly gelled silk fibroin solution is prepared.

[0038] The solution was diluted to 0.5%, poured into the above-mentioned shell conduit, and placed at 37° C. for 2 hours to induce gelation.

[0039] Insert the catheter vertically into the insulation material, along the axial direction of the catheter at a speed of 1×10 -6 m / s immersed in liquid nitrogen for directional freezing;

[0040] The frozen catheter is placed in a lyophilizer for freeze-drying, and aligned silk fibroin nanofibers are formed inside the collagen catheter to form a nerve catheter with the silk fibroin nanofiber orientation guiding function.

Embodiment 3

[0042] The 2% collagen solution was poured into the nerve conduit mold, frozen at -80° C. for 24 hours, and then freeze-dried to obtain the outer collagen conduit.

[0043] According to the method disclosed in patent CN106421902A, a rapidly gelled silk fibroin solution is prepared.

[0044] The silk fibroin solution was diluted to 0.01%, poured into the above-mentioned shell conduit, and placed at 37° C. for 24 hours to induce gelation.

[0045] Insert the catheter vertically into the insulation material, along the axial direction of the catheter at a speed of 1×10 -4 m / s immersed in liquid nitrogen for directional freezing;

[0046] The frozen catheter is placed in a lyophilizer for freeze-drying, and aligned silk fibroin nanofibers are formed inside the collagen catheter to form a nerve catheter with the silk fibroin nanofiber orientation guiding function.

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Abstract

The invention discloses a nerve conduit with a silk fibroin nanofiber directional guiding function. The nerve conduit comprises a core layer and a shell layer, wherein the shell layer is a degradable-polymer cylindrical hollow tubular porous conduit, and the core layer is formed by oriented silk fibroin nanofibers. The invention further discloses a preparation method of the nerve conduit. The preparation method comprises steps as follows: 1), the shell layer conduit is filled with a fibroin solution, and the fibroin solution is induced to be gelatinized; 2), the conduit is put in a liquid nitrogen environment for directional freezing, and directional silk fibroin nanofibers are formed in the core layer. The preparation method is simple to operate, and the prepared nerve conduit is good inbiocompatibility and is biodegradable; the nanofibers are directionally arranged in the conduit, growth factors can be loaded in the conduit, so that physical guiding signals and biological stimulating signals are provided for nerve cell growth, and conduits in different sizes can be prepared according to actual conditions for repair of spinal cord and peripheral nerve injury.

Description

technical field [0001] The invention relates to the technical field of nerve conduits, in particular to a nerve conduit containing silk fibroin nanofiber orientation guiding function and a preparation method thereof. Background technique [0002] The repair of nerve damage is an unresolved clinical problem. The use of biodegradable materials to develop nerve guide catheters with the function of promoting nerve regeneration is a research hotspot in the field of tissue engineering. At present, the nerve conduit materials that have been studied more are mainly hollow tubular structures. Compared with the autologous nerves, there is still a large gap in the repair effect of this kind of artificial nerve grafts. One of the important reasons is that in the process of nerve regeneration, due to the lack of suitable The physical guidance signal of the neuron will lead to the migration of neurons and the regeneration and extension of nerve fiber bundles, which will be misplaced or e...

Claims

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Application Information

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IPC IPC(8): A61L27/22A61L27/20A61L27/24A61L27/56A61L27/58
CPCA61L27/20A61L27/222A61L27/227A61L27/24A61L27/56A61L27/58A61L2430/32C08L89/00
Inventor 尤仁传王露萍李秀芳徐安长张强闫书芹
Owner WUHAN TEXTILE UNIV
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