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Application of mir-146a-5p in the treatment of visceral hypersensitivity in irritable bowel syndrome

1. The technology of mir-146a-5pagomir and irritable bowel syndrome, which is applied in the medical field, can solve the problems of intestinal permeability, serotonin signaling pathway and abnormal visceral or body hypersensitivity, so as to reduce the risk of irritable bowel syndrome. Effects of Visceral Hypersensitivity in Shock Syndrome

Active Publication Date: 2020-03-27
SHANDONG UNIV QILU HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, studies have found that microRNA is abnormally expressed in patients with irritable bowel syndrome (IBS), which leads to abnormalities in intestinal permeability, serotonin signaling pathway, and visceral or somatic hypersensitivity
There are no relevant reports on the diagnosis and / treatment of mir-146a-5p in visceral hypersensitivity in irritable bowel syndrome disease

Method used

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  • Application of mir-146a-5p in the treatment of visceral hypersensitivity in irritable bowel syndrome
  • Application of mir-146a-5p in the treatment of visceral hypersensitivity in irritable bowel syndrome
  • Application of mir-146a-5p in the treatment of visceral hypersensitivity in irritable bowel syndrome

Examples

Experimental program
Comparison scheme
Effect test

Embodiment approach

[0028] Visceral hypersensitivity refers to the enhanced sensitivity and responsiveness of visceral tissues to various mechanical and chemical stimuli, including visceral hyperalgesia and allodynia, and the volume and pressure threshold of the colorectum are significantly reduced when dilated stimuli are stimulated. At present, the mechanism of visceral hypersensitivity in IBS has not been fully clarified, which may include increased sensitivity of visceral receptors, abnormal sensory afferent nerves, and increased excitability of central visceral sensory neurons. In addition, endocrine and various neurotransmitter abnormalities, intestinal microorganisms and Low-grade inflammation is also involved in the formation of visceral hypersensitivity in IBS.

[0029] Agomir is a specially labeled and chemically modified double-stranded small RNA that regulates the biological functions of target genes by simulating endogenous miRNA.

[0030] In order to make the above and other objects...

Embodiment 1

[0031] Example 1 Detection of serum mir-146a-5p expression in patients with irritable bowel syndrome

[0032]The included research subjects included 5 patients with irritable bowel syndrome (irritable bowel syndrome), all of whom were visiting the Department of Gastroenterology at Qilu Hospital of Shandong University (visceral hypersensitivity state), and a total of 5 healthy volunteers were included in the healthy control group. The diagnostic criteria of diarrhea-predominant irritable bowel syndrome refer to the Rome III diagnostic criteria. Exclusion criteria were: patients with other diseases that caused diarrhea (tumor, thyroid disease, celiac disease, etc.); patients with a history of abdominal surgery or alarm symptoms (anemia, gastrointestinal bleeding, obvious weight loss, abdominal mass); patients with blood coagulation Abnormal function or severe organic disease affecting heart, liver and kidney function; pregnant or breastfeeding. Take 3ml of venous blood from 10 ...

Embodiment 2

[0034] Example 2 Establishment of mouse irritable bowel syndrome disease model and agomir intervention

[0035] The mice were fasted for 24 hours, and the mice were anesthetized by intraperitoneal injection of 1% pentobarbital (0.2ml / only), and the anal tube was inserted into the anus of the mice at one time, and TNBS (80mg / kg) was injected once at about 5cm from the anus as an enema After forming a model, the blank control group was given an enema with equal volume of normal saline. After the enema, the mice were turned upside down for about 30 minutes to prevent the liquid from flowing out. The Agomir intervention method is to start hsa-mir-146a-5p agomir (3nmol / bird) enema seven days after TNBS modeling, and enema once every three days. The disease model control group and the blank control group are given an equal volume of normal saline enema. On the 28th day, the colon dilatation test was performed to evaluate visceral hypersensitivity, and then the mice were sacrificed t...

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Abstract

The invention discloses application of mir-146a-5p to treating visceral hypersensitivity of irritable bowel syndrome. The mir-146a-5p is low in expression in peripheral serum of patients with visceralhypersensitivity of irritable bowel syndrome and intestinal mucosa of disease model mice with irritable bowel syndrome; meanwhile, after the disease model mice of irritable bowel syndrome receive intraperitoneal injection of hsa-mir-146a-5p agomir to achieve high expression of mir-146a-5p, the visceral sensitivity of the disease model mice is significantly reduced, so that the effectiveness of mir-146a-5p on reducing visceral hypersensitivity of the irritable bowel syndrome can be proved, and an effective way for treating visceral hypersensitivity of irritable bowel syndrome can be provided.

Description

technical field [0001] The invention belongs to the field of medical technology, and specifically relates to the application of non-coding small RNA gene mir-146a-5p, especially the application of mir-146a-5p in the preparation of medicine for treating visceral hypersensitivity in patients with irritable bowel syndrome. Background technique [0002] Irritable bowel syndrome (irritable bowel syndrome, IBS) is a functional gastrointestinal disease characterized by abdominal pain or discomfort accompanied by changes in bowel habits but without morphological and biochemical changes. One of the most common types of diseases in digestive system diseases has become an important factor affecting the quality of life of patients and increasing the social and economic burden. About 10%-20% of adults and adolescents worldwide have IBS symptoms, and most of them are women. [0003] A number of studies have shown that about 30% to 40% of IBS patients have symptoms of hypersensitivity to ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K48/00A61K31/7105A61P1/00
CPCA61K31/7105
Inventor 李延青李竹青左秀丽李理想
Owner SHANDONG UNIV QILU HOSPITAL
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