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Microbe flocculating agent and preparation method and application thereof

A microbial flocculant and floc-producing bacteria technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of high cost and limited application of chemical agents, and achieve the goal of improving raw material utilization and production efficiency Effect

Inactive Publication Date: 2018-04-20
卢梅雅
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The biggest feature of the chemical phosphorus removal system is that the phosphorus removal effect can be controlled by controlling the dosage of chemicals, but the cost of chemical agents is usually high, which limits its application in practical projects

Method used

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  • Microbe flocculating agent and preparation method and application thereof
  • Microbe flocculating agent and preparation method and application thereof
  • Microbe flocculating agent and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] The preparation method of a kind of microbial flocculant of the present embodiment comprises:

[0045] 1. Activated bacteria

[0046] (1) Inoculate Rhizopus microsporus (Rhizopus microsporus) CGMCC3.3608 in sterilized bran medium, and culture it at 28°C for 5 days, and the number of spores of Rhizopus microsporus in the culture exceeds 10 8 When individual / gram culture, obtain microsporin rhizopus agent, stand-by.

[0047] (2) Streptomyces parvus CGMCC4.5927 was inoculated in the sterilized Gaoshi No. 1 slant medium, and after culturing at 30°C for 7 days, inject sterile water with glass beads into the slant of the test tube, After fully shaking, filter into a single spore suspension, use photoelectric turbidimetry to detect the number of spores, and make the number of spores 10 9 individual / mL spore suspension for use.

[0048] (3) Inoculate Clostridium pasteurianum (Clostridium pasteurianum) CGMCC1.208 in a sterilized 250mL anaerobic bottle containing 100mL DCB-1 m...

Embodiment 2

[0058] The preparation method of a kind of microbial flocculant of the present embodiment comprises:

[0059] 1. Activated bacteria

[0060] (1) Inoculate Aspergillus tubingensis (Aspergillus tubingensis) CGMCC3.3292 in sterilized bran medium and culture it at 28°C for 5 days. The number of spores of Aspergillus tubingensis in the culture exceeds 10 8 Individual / gram culture, obtain Aspergillus tubingensis agent, stand-by.

[0061] (2) Inoculate Streptomyces diastaticus subsp.ardesiacus CGMCC4.1682 in the sterilized Gaoshi No. 1 slant medium, cultivate it at 30°C for 7 days, and inject the tape into the slant of the test tube. Sterile water with glass beads, after fully shaking, filtered into a single spore suspension, the number of spores was detected by photoelectric turbidimetry, and the number of spores was made to be 10 9 individual / mL spore suspension for use.

[0062] (3) Inoculate Clostridium pasteurianum (Clostridium pasteurianum) CGMCC1.208 in a sterilized 250mL a...

Embodiment 3

[0071] The preparation method of a kind of microbial flocculant of the present embodiment comprises:

[0072] 1. Activated bacteria

[0073] (1) Inoculate Penicillium montanense (Penicillium montanense) CGMCC 3.6588 in the sterilized bran medium and culture it at 28°C for 5 days. The number of spores of Penicillium montanense in the culture exceeds 10 8 Individual / gram culture, obtain Penicillium montana fungicide, stand-by.

[0074] (2) Inoculate Streptomyces griseorubens CGMCC4.5959 in the sterilized Gaoshi No. 1 slant medium, and after culturing at 30°C for 7 days, inject sterile glass beads with glass beads into the slant of the test tube. Water, after fully shaking, is filtered into a single spore suspension, and the number of spores is detected by photoelectric turbidimetry, and the number of spores is made to be 10 9 individual / mL spore suspension for use.

[0075] (3) Inoculate Clostridium pasteurianum (Clostridium pasteurianum) CGMCC1.208 in a sterilized 250mL anae...

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Abstract

The invention discloses a microbe flocculating agent and a preparation method and an application thereof. The preparation method comprises the following steps: 1) flocculant-producing bacteria can beinoculated in a crude fiber raw material, aerobic fermentation is carried out, and a primary fermentation object is obtained; 2) clostridium pasteurianum CGMCC1.208 is inoculated in the primary fermentation object, anaerobic fermentation is carried out, and a secondary fermentation object is obtained; 3) after the water content of the secondary fermentation object is reduced to 25-30%, myxobacteria is inoculated in the secondary fermentation object, and a third fermentation matrix with the water content being lower than 40% and a pH value being 6-8 is obtained; 4) aerobic fermentation is carried out, and a third fermentation object is obtained; and 5) the third fermentation object is extracted by using ethyl acetate, leaching liquor is taken, filtering is carried out to take clear liquid,and drying is carried out to obtain the microbe flocculating agent. The method employs the flocculant-producing bacteria-clostridium pasteurianum-myxobacteria for successively performing aerobic fermentation, anaerobic fermentation and aerobic fermentation on the crude fiber raw material, and the obtained flocculating agent has excellent phosphate removing effect on phosphor enriched sewage.

Description

technical field [0001] The invention relates to the technical field of sewage treatment, in particular to a microbial flocculant and its preparation method and application. Background technique [0002] At present, in the process of urban sewage treatment, not only COD and SS are required to be taken out, but also higher requirements are put forward for phosphorus removal and nitrogen removal. However, in rural areas, there are still many people who use phosphorus-rich washing powder because of weak environmental awareness and greed for cheap, which leads to the situation of phosphorus pollution in rural rivers is not optimistic. [0003] Sewage phosphorus removal technology can be divided into two categories: biological phosphorus removal and chemical phosphorus removal. Among them, the essence of biological phosphorus removal is to excessively absorb phosphorus in wastewater under aerobic conditions through phosphorus accumulating bacteria and accumulate it in cells, and ...

Claims

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Application Information

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IPC IPC(8): C12P39/00C12P1/04C12P1/02C02F3/34C12R1/145C12R1/465C12R1/785C12R1/885C12R1/66C12R1/80C12R1/845C02F101/10
CPCC02F3/34C02F3/348C02F2101/105C12P1/02C12P1/04C12P39/00
Inventor 卢梅雅
Owner 卢梅雅
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