Composite microbial agent for treating black and odor water bodies, obtaining method and use method thereof

A technology of compound microbial agent and black and odorous water body, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problem of low efficiency of microbial agent treatment, poor specificity of river and pit sewage treatment, etc. problem, to achieve the effect of high efficiency and specificity

Active Publication Date: 2018-04-20
BOYUAN ECOLOGICAL RESTORATION (BEIJING) CO LTD +3
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Problems solved by technology

[0004] Aiming at the problems that the treatment efficiency of the existing microbial agents used in the treatment of black and odorous water bodies is not high, and the specificity of the treatment of sewage in rivers and pits is poor, the present invention ...

Method used

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Embodiment Construction

[0036] The black and odorous water body was collected from a pond in Qingguang Town, Beichen District, Tianjin, and the strains were isolated and screened from the raw water:

[0037] (1) Specific screening medium to screen Bacillus. Leave the sewage (derived from black and odorous water) for 24 hours, mix the supernatant with sterile water, and press 10 -4 、10 -5 and 10 -6 Doubling gradient dilution to make bacterial suspension, add it to specific screening medium (liquid), and cultivate for 24 hours; dilute the expansion culture solution by 10 3 、10 4 、10 5 and 10 6 double, spread evenly on the improved specific screening medium (solid) plate, and cultivate for 12 hours; pick a single colony, and repeatedly carry out liquid culture and streak separation on a single strain to obtain pure strains of Bacillus megaterium F5 and thermophilic fatty soil Bacillus F32; LB test tube slant and cryopreserved.

[0038] Specific screening medium: per 1000mL water, glucose 2g, star...

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Abstract

The embodiments of the present invention provide a composite microbial agent for treating black and odor water bodies, wherein the composite microbial agent is mainly used for treating black and odorwater bodies of urban river courses and town pit ponds, and comprises Pseudomonas sp. AW0H4, Bacillus megaterium AW0F5, Alcaligenes faecalis, Bacillus pumilus and Geobacillus stearothermophilus. According to the present invention, the single strain is obtained through a fixed screening method, wherein the microbial biomass achieves 1*10<9> cfu/mL after the single strain is activated and concentrated, and the composite microbial agent is prepared by mixing according to a ratio of 2:2:2:1:1 or 2:2:1:1:1; the storage method comprises: adding a glycerol protection agent to the single strain concentrated bacterial liquid or the composite microbial agent, and freezing; and the composite microbial agent has high specificity for black and odor water bodies, and the metabolic functions of the strains in the composite microbial agent are highly complementary to each other.

Description

technical field [0001] The invention relates to the technical field of treatment of black and odorous water bodies, in particular to a composite microbial agent for treatment of black and odorous water bodies, its obtaining method and its use method. Background technique [0002] In recent years, excessive pollution in urban rivers and urban pits and ponds has led to an imbalance in oxygen supply and consumption in water bodies. Under anoxic or even anaerobic conditions, pollutants in water bodies are transformed and produce odorous substances such as ammonia nitrogen, hydrogen sulfide, and volatile organic acids, as well as iron and manganese. Black substances such as sulfides appear black and odorous, which not only destroys the original ecological functions of the water body, but also seriously affects the health and normal life of surrounding residents. [0003] The treatment of black and odorous water mainly adopts technologies such as physical aeration, ecological engi...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34C12R1/38C12R1/11C12R1/05C12R1/07
CPCC02F3/34C02F2209/08C02F2209/14C12N1/20C12N1/205C12R2001/11C12R2001/38
Inventor 吴文韬张伦梁张会敏汤瑶李维尊
Owner BOYUAN ECOLOGICAL RESTORATION (BEIJING) CO LTD
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