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Antibiotic detector based on nanogold-aptamer structure and detection method

A technology of detection device and detection method, which is applied in the direction of measurement device, color/spectral characteristic measurement, and analysis by chemical reaction of materials, etc. Influence of ambient light, effect of improving measurement accuracy

Active Publication Date: 2018-04-17
TAIYUAN UNIV OF TECH
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Problems solved by technology

[0007] Although the above-mentioned methods can detect the concentration of antibiotics, large-scale instruments are used in the final detection, such as ultraviolet-visible spectrophotometer, fluorescence spectrometer, etc. The detection process is complicated and long, and it is impossible to realize on-site rapid detection

Method used

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  • Antibiotic detector based on nanogold-aptamer structure and detection method
  • Antibiotic detector based on nanogold-aptamer structure and detection method
  • Antibiotic detector based on nanogold-aptamer structure and detection method

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example 4

[0047] Experiment 4. Detection of different concentrations of SDM

[0048] According to the above-mentioned optimized sequence, different concentration antibiotic detection experiments were carried out, and the ultraviolet-visible light absorption spectrum of the solution was measured by a spectrophotometer.

[0049] The results show that: if figure 2 As shown, with the gradual increase of the concentration of sulfonamide antibiotics in the solution, the position of the maximum absorption peak of the absorption spectrum gradually red-shifted, and reached a stability at 5 ppm, which proved that the method can realize the quantitative detection of SDM.

[0050] Although the above only describes the detection principle and preparatory experiments of the antibiotic sulfadimethoxine (SDM), for other antibiotics, the concentration can also be achieved through the corresponding aptamer solution and gold nanoparticles. Detection, for example, for kanamycin, its aptamer chain is: 5'-...

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Abstract

The invention discloses a sulfonamide antibiotic detector based on a nanogold-aptamer structure and a detection method. The detector comprises an intelligent device, a multi-flux detection unit, and an optical hardware unit, the multi-flux detection unit comprises a guide tube holder and a plurality of glass guide tubes arranged in a line on the guide tube holder, the glass guide tubes are respectively used for containing reaction solution and to-be-detected solution or reaction solution and standard solutions containing different concentrations of sulfonamide antibiotics, the interior of theoptical hardware unit is provided with an accommodating space for the placement of the multi-flux detection unit, the intelligent device is used for photographing the liquids in the multi-flux detection unit and obtaining the wavelengths of the corresponding solutions according to RGB (Red, Green and Blue) values of pictures, so that the concentration of sulfonamide antibiotic in the to-be-detected solution is obtained, and the optical hardware unit is used for providing uniform illumination for the photographing of the intelligent device. According to the invention, on-site real-time on-linerapid detection on antibiotics can be realized, and the sulfonamide antibiotic detector based on a nanogold-aptamer structure and the detection method can be popularized in various fields such as medical diagnosis, food safety and environmental monitoring.

Description

technical field [0001] The invention belongs to the field of food safety and water pollution detection, specifically relates to the field of on-site rapid real-time online detection of antibiotics in water and food, and proposes an antibiotic detection device and method based on a nano-gold-aptamer structure. Background technique [0002] In recent years, the problem of environmental pollution has been widely concerned by people, especially the residue of antibiotics has become the focus of attention. Antibiotics are specific drugs for the treatment of infectious diseases. They are widely used in human medicine and treatment of animal diseases. They have the characteristics of strong antibacterial properties and a large amount of use. Due to the large and unreasonable use of antibiotics, antibiotics have entered water bodies and polluted the water environment. When antibiotics enter the body and accumulate in the body, people will develop resistance to antibiotics, causing ...

Claims

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Application Information

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IPC IPC(8): G01N21/01G01N21/27G01N21/78
CPCG01N21/01G01N21/278G01N21/78
Inventor 李晓春王乐张校亮于化忠
Owner TAIYUAN UNIV OF TECH
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