Novel methylation library-establishing kit and application thereof
A kit and methylation technology, applied in the field of molecular biology, can solve the problems of expensive enzymes, no linking ability, and reduced linking efficiency
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[0238] 1. Preparation of Methylation Library Construction Kit
[0239] (1) Synthesis of double-stranded adapters with sticky ends in the methylation library construction kit:
[0240] The sticky end adapter is a double-stranded linker with a sticky end, one end of the sticky end adapter is a first sticky end, and the first sticky end includes at least a random sequence.
[0241] 1. As an example, use Primer1 and Primer2 to synthesize sticky end adapters,
[0242] Primer1: 5'-AGATCGGAAG-3'-bioten (SEQ ID NO.1);
[0243] Primer2: 5'-CTTCCGATCTNNNNNN-3' (SEQ ID NO.2); wherein, NNNNNN represents a random sequence, usually the length of the random sequence is 2-30nt, preferably 4-20nt, more preferably 5-10nt .
[0244] 2. Add the configuration reaction solution according to Table 1:
[0245] Table 1
[0246] Reagent
Volume (ul)
Primer1 (10μM)
1
Primer2 (10μM)
1
7
5M NaCl
1
total capacity
10
[0247]...
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