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Biological agent for remediation of organic polluted soil and its preparation and use method

A biological agent and soil remediation technology, applied in the field of contaminated soil remediation and biological agents, can solve the problems of difficult natural degradation, secondary pollution, application restrictions, etc., and achieve the effect of low price

Active Publication Date: 2019-12-03
CHINESE RES ACAD OF ENVIRONMENTAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] However, the application of chemically synthesized surfactants is limited due to the source of raw materials, price, product performance, etc., especially the possibility of secondary pollution and difficulty in natural degradation.

Method used

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  • Biological agent for remediation of organic polluted soil and its preparation and use method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] A kind of biological preparation for the remediation of contaminated soil, the composition includes biosurfactant and solubilizer; The biosurfactant is composed of glycolipid, glycoside, cellulose, soybean oil, saponin, dextrin, starch, lignin Any two or more of them are mixed with water, and the content of any other component in the biosurfactant except water in the biological preparation is 2~80g / L, preferably 2~45g / L, more preferably 15 ~ 35g / L; the solubilizer is any one or more of sodium chloride, hydrochloric acid, sodium hydroxide, phosphate, nitrate; the pH value of the biological preparation is 6 ~ 9, the The salt concentration in the biological preparation is 2-50mol / L; the surface tension value of the biological preparation is 15-45mN / m, and the critical micelle concentration of the biological preparation is 20-800mg / L.

[0020] The biosurfactant is preferably composed of glycolipids, glycosides mixed with water, or at least one of glycolipids, glycosides and...

Embodiment 2

[0026] This embodiment is an improvement made on the basis of Embodiment 1. For the same part as Embodiment 1 in this embodiment, please refer to the content disclosed in Embodiment 1 for understanding, and the content disclosed in Embodiment 1 should also be used as this embodiment. The content of the example will not be repeated here.

[0027] Prepare a biosurfactant mixed with glycolipid, soybean oil, and water, add a solubilizer to adjust the pH to 8.5, and ensure that the salt concentration is 2.5mol / L to obtain the biological preparation Sole-A, in which the concentrations of glycolipid and soybean oil are respectively 15g / L and 35g / L, diluted two hundred times with local well water and river water on site.

[0028] Prepare a biosurfactant mixed with saponin, lignin, and water, add a solubilizer to adjust the pH=9.0, and ensure that the salt concentration is 2.5mol / L to obtain the biological preparation Sole-B, wherein the concentrations of saponin and lignin are 15g res...

Embodiment 3

[0036] Containing chlorobenzene and toluene are respectively 61mg / kg and 85mg / kg pesticide production site soil 10 grams, in 500ml Erlenmeyer flask. The 5 kinds of Sole preparations in Example 1 are diluted 150 times with the surface water near the site, each 50ml is added into 5 500ml Erlenmeyer flasks filled with 10 grams of polluted soil respectively, shaken, and the soil is taken for testing, the removal rate of chlorobenzene and toluene They reached 35%-75% and 41%-81% respectively. Keep the soil moist and agitate intermittently. After 30 days, the removal rate of chlorobenzene and toluene in the soil is improved. The best preparation can reduce the concentration of chlorobenzene and toluene by 85% and 93%, respectively.

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Abstract

The invention discloses a biological agent for contaminated soil restoration and preparing and using methods thereof. The biological agent is composed of a biosurfactant and a solubilizer, the biosurfactant is formed by mixing any two or more of glycolipid, glucoside, cellulose, soybean oil, saponin, dextrin, starch and lignin with water, and content of the above ingredients in the biological agent is 2-80g / L; the solubilizer is one or multiple of sodium chloride, hydrochloric acid, sodium hydroxide, phosphate and nitrate. PH value of the biological agent is 6-9, concentration of salt is 2-50mol / L, and surface tension value is 15-45mN / m. The biological agent extracts hydrophobic contaminants in soil through dispersing, solubilizing and emulsifying action and activates growth and activity of indigenous microorganisms so as to promote degradation of organic contaminants and can be degraded naturally, thereby preventing secondary pollution.

Description

technical field [0001] The invention relates to the technical field of environmental protection, in particular to a biological preparation for remediation of polluted soil and methods for its preparation and use. The biological preparation can extract hydrophobic organic matter in soil and promote its biodegradation. Background technique [0002] For a long time, the spillage and leakage of organic substances such as oil and pesticides will cause soil pollution. After leakage, these pollutants first infiltrate into the vadose zone, migrate under the interaction of capillary force and gravity, and exist as pollution sources for a long time, seriously damaging the original soil environment. , poison soil microorganisms, reduce soil quality, affect agricultural production, and even threaten groundwater safety and affect human health. [0003] The role of surfactants in the remediation of soil oil pollution is mainly through their own adsorption behavior on the soil, forming dif...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C09K17/40B09C1/10
CPCB09C1/10C09K17/40
Inventor 刘俐
Owner CHINESE RES ACAD OF ENVIRONMENTAL SCI
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