Preparation method and application of pleurotus eryngii bran fermented feed
A technology of Pleurotus eryngii fungus chaff and fermented feed, which is applied in the shaping or processing of animal feed, application, animal feed, etc. It can solve the problems of unfavorable ruminant rumination, increased production workload, and long feed production cycle, etc., to achieve The effect of shortening the slaughtering time, significant economic benefits, and reducing feeding costs
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[0022] The present invention will be further described below in conjunction with embodiment. The examples are given only to illustrate the invention.
[0023] (1) Preparation of composite microbial agent
[0024] 1.1. Medium
[0025] (1) LB liquid medium (g / L): Take 10 g of peptone, 5 g of yeast extract, and 5 g of sodium chloride, dissolve in 1 L of distilled water, and sterilize at 121 ° C for 30 min.
[0026] (2) PDA liquid medium (g / L): Peel potatoes, take 200g and cut into pieces, boil for 30min, then filter with gauze, add 20g of glucose to the filtrate, make up distilled water to 1000mL after melting, and sterilize at 121°C 30min.
[0027] (3) MRS liquid medium (g / L): take beef extract 10.0g, peptone 10.0g, yeast extract 5.0g, glucose 20.0g, Tween 80 1.0g, dipotassium hydrogen phosphate 2.0g, sodium acetate 5.0g, Dissolve 2.0g of triammonium citrate, 0.2g of magnesium sulfate, and 0.05g of manganese sulfate in 1L of distilled water, and sterilize at 121°C for 20min....
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