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DNA (deoxyribonucleic acid) preservation solution for oral swabs and method for preparing DNA preservation solution

A preservation solution and swab technology, applied in the field of oral swab DNA preservation solution and its preparation, can solve the problems of short preservation time, unsanitary, easy to breed bacteria, etc. Effect

Inactive Publication Date: 2018-04-13
南京普维康生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the storage of oral swabs is generally sealed in a marked envelope, which is not hygienic and prone to bacteria; and the storage time is short

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] A buccal swab DNA preservation solution, comprising the following raw materials in weight-to-volume ratio: Tris 11g / 100mL, sodium alginate 8g / 100mL, tripotassium phosphate 3g / 100mL, zinc gluconate 4g / 100mL, 0.1mol / L disodium edetate 8g / 100mL, L-carnitine 3g / 100mL, valine 0.01g / 100mL, natural preservative 0.05g / 100mL, vitamin e 0.5g / 100mL, normal saline 30g / 100mL, Stevioside 1g / 100mL, sodium polystyrene sulfonate 1g / 100mL, sodium dihydrogen phosphate 4g / 100mL; the solvent is deionized water with a pH value of 6.8. The pH value is adjusted by hydrochloric acid.

[0018] A kind of preparation method of buccal swab DNA preservation solution, take trishydroxymethylaminomethane, sodium polystyrene sulfonate, valine, vitamin E, disodium edetate, natural preservatives, physiological saline and mix, Obtain the preservation base liquid; mix sodium alginate, tripotassium phosphate, zinc gluconate, L-carnitine, stevioside, and sodium dihydrogen phosphate with the preservation bas...

Embodiment 2

[0021] A buccal swab DNA preservation solution, comprising the following raw materials in weight-to-volume ratio: Tris 15g / 100mL, sodium alginate 10g / 100mL, tripotassium phosphate 7g / 100mL, zinc gluconate 8g / 100mL, 0.15mol / L disodium edetate 10g / 100mL, L-carnitine 5g / 100mL, valine 0.1g / 100mL, natural preservative 0.1g / 100mL, vitamin e1.5g / 100mL, normal saline 50g / 100mL, Stevioside 2g / 100mL, sodium polystyrene sulfonate 3g / 100mL, sodium dihydrogen phosphate 8g / 100mL; the solvent is deionized water with a pH value of 9. The pH value is adjusted by hydrochloric acid.

[0022] A kind of preparation method of buccal swab DNA preservation solution, take trishydroxymethylaminomethane, sodium polystyrene sulfonate, valine, vitamin E, disodium edetate, natural preservatives, physiological saline and mix, Obtain the preservation base liquid; mix sodium alginate, tripotassium phosphate, zinc gluconate, L-carnitine, stevioside, and sodium dihydrogen phosphate with the preservation base ...

Embodiment 3

[0025] A buccal swab DNA preservation solution, comprising the following raw materials in weight-to-volume ratio: Tris 12g / 100mL, sodium alginate 9g / 100mL, tripotassium phosphate 5g / 100mL, zinc gluconate 6g / 100mL, 0.12mol / L disodium edetate 9g / 100mL, L-carnitine 4g / 100mL, valine 0.03g / 100mL, natural preservative 0.07g / 100mL, vitamin e 0.8g / 100mL, normal saline 40g / 100mL, Stevia 1.6g / 100mL, sodium polystyrene sulfonate 2.2g / 100mL, sodium dihydrogen phosphate 5g / 100mL. The solvent is deionized water with a pH value of 7.2.

[0026] A kind of preparation method of buccal swab DNA preservation solution, take trishydroxymethylaminomethane, sodium polystyrene sulfonate, valine, vitamin E, disodium edetate, natural preservatives, physiological saline and mix, Obtain the preservation base liquid; mix sodium alginate, tripotassium phosphate, zinc gluconate, L-carnitine, stevioside, and sodium dihydrogen phosphate with the preservation base liquid, and adjust the pH value of the bucca...

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Abstract

The invention discloses DNA (deoxyribonucleic acid) preservation solution for oral swabs. The DNA preservation solution comprises, by weight-to-volume ratio, trihydroxymethyl aminomethane, sodium alginate, tripotassium phosphate, zinc gluconate, ethylene diamine tetraacetic acid disodium, L-carnitine, valine, natural preservatives, vitamin e, normal saline, stevioside, sodium polystyrene sulfonateand sodium dihydrogen phosphate. Deionized water is used as a solvent for the DNA preservation solution. The DNA preservation solution has the advantages that the DNA preservation solution is preservation solution pertinently for the oral swabs, and oral cells can be effectively preserved by the DNA preservation solution; the DNA preservation solution is hygienic, and bacterium breeding can be prevented; the preservation time is longer than 3-5 times the preservation time of envelopes; a method for preparing the DNA preservation solution is simple and has a good social value.

Description

technical field [0001] The invention relates to a DNA preservation solution, in particular to an oral swab DNA preservation solution and a preparation method thereof. Background technique [0002] The oral cell sampling steps include: 1) Tear off the outer packaging of the oral swab, and carefully take out the oral swab (note: hands cannot touch the swab during the entire sampling process). 2) Hold the handle, insert the swab into the left oral cavity, make the swab head fully touch the mucous membrane on the inside of the left cheek / left upper and lower gums, and rub up and down with the strength of brushing teeth. At the same time, rotate the swab to let the swab The child's head fully contacts the oral mucosa, and repeats this action for 1 minute. 3) Use the same method to sample the second swab at the mucosa of the right cheek / right upper and lower gums. 4) Put the swab stained with oral exfoliated cells in a clean and ventilated place for at least one hour until it dr...

Claims

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Application Information

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IPC IPC(8): A01N1/02
CPCA01N1/021A01N1/0215
Inventor 徐朋朋李爽许银平
Owner 南京普维康生物科技有限公司
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