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Genetic biochemical site marker for cerebral ischemia inbred line meriones unguiculatus and application of biochemical site marker

A long-clawed gerbil and inbred line technology is applied in the field of genetic and biochemical site markers, and can solve the problem that the genetic and biochemical site marker detection method of inbred gerbils has not been established, and the genetic and biochemical site markers of gerbils have not been determined. And other issues

Active Publication Date: 2018-04-03
ACADEMY OF MILITARY MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0011] However, no genetic and biochemical locus markers have been identified for gerbils, nor have methods for detecting genetic and biochemical locus markers for inbred gerbils been established

Method used

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  • Genetic biochemical site marker for cerebral ischemia inbred line meriones unguiculatus and application of biochemical site marker
  • Genetic biochemical site marker for cerebral ischemia inbred line meriones unguiculatus and application of biochemical site marker
  • Genetic biochemical site marker for cerebral ischemia inbred line meriones unguiculatus and application of biochemical site marker

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0143] Example 1 Detection of Glucose-6-phosphate dehydrogenase-1 (Glucose-6-phosphate dehydrogenasc-1, Gpd1)

[0144] Supernatant samples from kidney and liver were tested separately.

[0145] Soak plates: Soak cellulose acetate plates in Tris-glycine pH 8.9 buffer, avoiding air bubbles on the plates.

[0146] Adding samples: Use a micro-sampler to add samples to the sample plate, not exceeding 10 μl per well.

[0147] Sample spotting: Take out the soaked plate, blot the remaining liquid in two layers of filter paper, immediately put it on the spotting plate of the super sampler, use the super sampler to sample on the sample plate, add the soaked cellulose acetate plate . Adding samples and spotting samples is a combination of super samplers.

[0148] Electrophoresis: Place the sampled cellulose acetate plate flatly on the filter paper bridge of the electrophoresis tank bracket (the sample point is facing down), the sample point is located at the cathode, pay attention tha...

Embodiment 2

[0153] The detection of embodiment 2 esterase-3 (esterase-3, Es3)

[0154] Soak plates: Soak cellulose acetate plates in Tris-glycine pH 8.9 buffer, avoiding air bubbles on the plates.

[0155] Adding samples: Use a micro-sampler to add samples to the sample plate, not exceeding 10 μl per well.

[0156] Sample spotting: Take out the soaked plate, blot the remaining liquid in two layers of filter paper, immediately put it on the spotting plate of the super sampler, use the super sampler to sample on the sample plate, add the soaked cellulose acetate plate . Wherein, adding sample plate and pointing sample plate are the combination of super sampler.

[0157] Electrophoresis: Place the sampled cellulose acetate plate flatly on the filter paper bridge of the electrophoresis tank bracket (the sample point is facing down), the sample point is located at the cathode, pay attention that the film is close to the stretched filter paper, cover the electrophoresis tank cover, Adjust th...

Embodiment 3

[0161] The detection of embodiment 3 carbonic anhydrase-2 (carbonic anhydrase-2, Car2)

[0162] Dip plate: soak acetate plate in NaC 2 h 3 o 2 -EDTA pH5.4 buffer solution (diluted 1:4) to avoid air bubbles on the membrane.

[0163] Adding samples: Use a micro-sampler to add samples to the sample plate, not exceeding 10 μl per well. Sample spotting: Take out the soaked membrane, blot the remaining liquid in two layers of filter paper, immediately put it on the sample spotting plate of the super sampler, use the super sampler to sample on the sample plate, and add the soaked cellulose acetate plate.

[0164]Electrophoresis: Place the sampled fiber acetate plate flatly on the filter paper bridge of the electrophoresis tank bracket (the sample point is facing down), the sample point is located at the anode, pay attention to the film sticking to the stretched filter paper, cover the electrophoresis tank cover, and adjust the voltage To 240V, switch on the electrophoresis instru...

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Abstract

The invention relates to a genetic biochemical site marker for cerebral ischemia inbred line meriones unguiculatus and application of the biochemical site marker. The genetic biochemical site marker for the cerebral ischemia inbred line meriones is selected from at least one of glucose-6-phosphate dehydrogenase-1, esterase-3, carbonic anhydrase-2, alkaline phosphatase-1, isocitrate dehydrogenase-1, malic enzyme-1, glucose phosphate translocase-1, renal catalase-2, peptidase-3, glucose phosphate isomerase-1, hemoglobin-beta chain, transferring, esterase-1, esterase-10, glycerol phosphate dehydrogenase-1, beta-glucuronidase-1, esterase-2, lactic dehydrogenase regulator-1, serum protein-1, amylase-1, esterase-6, esterase-8, esterase-9, esterase-4, catalase-1 and esterase-12.

Description

technical field [0001] This application relates to the genetic and biochemical locus markers of cerebral ischemia inbred strain long-clawed gerbils, especially its application in judging cerebral ischemia inbred strains of long-clawed gerbils and the cerebral ischemia inbred long-claw Gerbil detection method. Background technique [0002] The inheritance of inbred animals has the characteristics of gene homogeneity, genetic stability, phenotype uniformity, individual uniqueness and wide distribution. The results of animal experiments are reliable, regular and repeatable, and are widely used in the field of biomedical research. [0003] At present, the methods for testing the genetic quality of experimental animals mainly include coat color gene testing, biochemical marker testing, immune marker gene testing, mandible bone morphometric analysis, and molecular marker testing. [0004] 1. Coat color gene testing method: this method is a traditional, intuitive morphological de...

Claims

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Application Information

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IPC IPC(8): G01N27/447
CPCG01N27/447
Inventor 王冬平陈振文马兰芝尚世臣黄斌李桂军
Owner ACADEMY OF MILITARY MEDICAL SCI
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