A kind of tissue culture rapid propagation method of Polygonatum multiflora

Abstract

The invention provides and relates to the technical field of plant tissue culture, and provides a tissue culture rapid propagation method of polygonatum multiflorum. The method sequentially comprisesthe following steps of performing sand storage; performing soaking by warm water being 35 to 40 DEG C; performing gibberellin treatment; performing pretreatment on the polygonatum multiflorum seeds soas to break the seed dormancy; inoculating the pretreated seeds into a seed cluster bud inducing culture medium; directly inducing the seeds into cluster buds, wherein the seed cluster bud inducing culture medium is prepared from 1.5 to 2.5mg / L of MS culture media 6-BA, 0.5 to 1.5mg / L of GA3, 6.5 to 7g / L of agar and 30 to 35g / L of sucrose, and the pH value is 5.5 to 6.0; dividing the cluster budsand performing propagation culture so as to expand the cluster bud quantity; performing rooting culture and domestication transplanting to obtain polygonatum multiflorum stock seedlings. The method provided by the invention has the advantages that the dormancy of the polygonatum multiflorum seeds can be effectively broken; the seed germination stage is shortened to five months; the effects of short propagation period and germination rate improvement by 85 to 90 percent are achieved.

Description

technical field The invention relates to the technical field of plant tissue culture, in particular to a method for tissue culture and rapid propagation of Polygonatum polyflora. Background technique Polygonatum cyrtonema (scientific name: Polygonatum cyrtonema Hua) is a perennial herb of the genus Polygonatum cyrtonema in the family Liliaceae. Oblong-lanceolate, umbel, perianth yellow-green, black berries, flowering from May to June, fruiting from August to October. The rhizome of Polygonatum multiflora can be used as medicine or as an ornamental plant. Polygonatum multiflora is a species of Polygonatum specified in the "Chinese Pharmacopoeia (2015)", which has the effects of invigorating qi and nourishing yin, strengthening the spleen, moistening the lungs, and nourishing the kidneys. In recent years, with the in-depth research on the efficacy of Polygonatum multiflora and the advent of health care products and medicines for Polygonatum multiflora, the market demand for...

AI Technical Summary

Problems solved by technology

Rhizome has very strong reproductive potential, can grow young shoots and root system, form new plant and carry out asexual reproduction, thereby prior art mostly uses the rhizome of Polygonatum polyflora as explant research, easily produces bunchy bud or heals. Injury tissue, thereby speeding up the incubation time and increasing the proliferation coefficient

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