Near-infrared detection method for lysine content in muscle freeze-dried powder of Yunnan native chickens

A technology of near-infrared detection and freeze-dried powder, which is applied in the field of chemical composition analysis of livestock and poultry products, can solve problems such as the lack of research content on leg muscles, and achieve the effects of expanding the scope of application, simple prediction models, and convenient use

Inactive Publication Date: 2018-03-30
YUNNAN AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the current research on the near-infrared analysis of chicken, it is basically the research on the breast muscle. Few scholars have done research on the mixed samples of the leg and breast muscle. In the production and consumption of chicken, the leg muscle is also a very important part of chicken. , so there is still a lack of research on leg muscles
However, there is no relevant report on the quantitative detection model of chicken near-infrared lysine

Method used

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  • Near-infrared detection method for lysine content in muscle freeze-dried powder of Yunnan native chickens
  • Near-infrared detection method for lysine content in muscle freeze-dried powder of Yunnan native chickens
  • Near-infrared detection method for lysine content in muscle freeze-dried powder of Yunnan native chickens

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0061] (1) Use 2 samples of leg muscle and breast muscle of Wuding chicken, Xinping Liming chicken, Nixi chicken, Wuliangshan black-bone chicken, and Jianchuan blue-and-white chicken, and 6 samples of fluffy chicken leg muscle and breast muscle respectively, and scan according to the spectrum Spectral scanning is required, and the scanning wavenumber range is 3996-10002cm -1 , the obtained original spectrum is subjected to SNV and Gapsegment(1#,15,7) spectral preprocessing, and the processed spectrum is as follows image 3 shown.

[0062] (2) Determination of the lysine content of the sample by liquid chromatography.

[0063] (3) Each sample 4050 ~ 9949cm -1 The absorbance value after pretreatment corresponding to the wave number was substituted into the model formula for calculation, and the predicted lysine content was obtained.

[0064] Table 5 Comparison table of sample lysine chemical detection value and model prediction value

[0065]

[0066]

[0067] It can b...

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Abstract

The invention relates to a near-infrared detection method for lysine content in muscle freeze-dried powder of Yunnan native chickens, and belongs to the technical field of poultry body composition detection. The method particularly comprises the following steps: 1) preparing leg muscle or pectoral muscle fresh samples of the Yunnan native chickens into freeze-dried powder, putting into a polyethylene self-sealing bag with the thickness being 6 filaments; 2) scanning a near-infrared spectrum of the samples, wherein the wavenumber range of spectrum scanning is 3996 to 10002 cm<-1> and the resolution is 8 cm<-1>,taking the single layer of the 6-filament self-sealing bag to be placed in front of a probe to remove background effects while scanning the spectral background, scanning each sample for 3 times, and taking the average value of the scanning as spectral data of the samples, wherein each spectrum has 1558 absorbance values; 3) after preprocessing the absorbance of the samples with SNV, then performing preprocessing by a Gap-segment-order derivative method; 4) substituting preprocessed absorbance data in the wavenumber range of 4050 to 9949 cm<-1> into a model formula, thus obtaining the lysine content of the samples. The near-infrared detection method for the lysine content in the muscle freeze-dried powder of the Yunnan native chickens has the advantages of high forecastingspeed, non-destructive samples, suitability for large-scale sample detection and the like.

Description

technical field [0001] The technical field of chemical composition analysis of livestock and poultry products of the present invention, in particular, relates to a near-infrared detection method for lysine content in Yunnan local chicken muscle freeze-dried powder. Background technique [0002] Lysine is a basic amino acid, which is an essential amino acid for both poultry and human body. It is the first limiting amino acid for human body and the second limiting amino acid for poultry. Its main function is for protein synthesis. In the "ideal amino acid" model of poultry, all amino acid requirements are referenced to lysine, so once the information on lysine requirements is wrong, everything else will be wrong, so lysine has always been an important ingredient in animal nutrition. research hotspots in the field. [0003] Yunnan Province is located in the southwestern border of China, near the Tropic of Cancer. The terrain is high in the north and low in the south, with a la...

Claims

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Application Information

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IPC IPC(8): G01N21/359G01N1/28
CPCG01N1/286G01N21/359
Inventor 陶琳丽张曦杨秀娟邓君明曹胜雄黄伟刘廷丹戚敏
Owner YUNNAN AGRICULTURAL UNIVERSITY
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