Method for synchronously detecting multiple antibiotics in polluted water
A technology of simultaneous detection and antibiotics, applied in measuring devices, instruments, scientific instruments, etc., can solve problems such as antibiotic pollution and farmland soil environmental pollution, and achieve the effect of low detection limit, high detection accuracy, and good practical application prospects.
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Embodiment 1
[0038] The mensuration of embodiment 1 standard curve, detection limit, limit of quantification and rate of recovery
[0039] (1) Preparation of antibiotic standard stock solution
[0040] Antibiotics to be tested include tetracycline (TC), 4-epitetracycline hydrochloride (E-TC), chlortetracycline (CTC), epitetracycline hydrochloride (E-CTC), oxytetracycline (OTC), 4- Epitropic oxytetracycline (E-OTC), penicillin (PCN), ampicillin (AMP), cefotaxime (CTX), neospiramycin (NSPM), spiramycin (SPM), clarithromycin ( CLR), Tylosin (TLY), Roxithromycin (NERY), Norfloxacin (NOR), Ciprofloxacin (CIP), Tilmicosin (TIL), Enrofloxacin (ENR) , ofloxacin (OFX), sulfamethazine (SMZ), sulfamethoxine (SMX), sulfamethizole (SML), sulfadiazine (SD) and sulfamethazine (SMN), a total of 24 kinds . Accurately weigh 0.010g of each drug, dissolve it with methanol and dilute it to a 10mL brown volumetric flask, mix well to obtain a stock solution with a mass concentration of 1000mg L-1, store it in...
Embodiment 2
[0064] The detection of 24 kinds of antibiotics in the livestock and poultry breeding waste water of embodiment 2
[0065] Detect a certain waste water body sample with the method and condition identical with embodiment 1, experimental procedure is as follows:
[0066] (1) Take 100mL sample of the measured pig farm wastewater at 5000r·min -1 Centrifuge for 10 min, take the supernatant and pass through a 0.45 μm filter membrane, and use 1mol L -1 hydrochloric acid to adjust the pH of the filtrate to 3.85, add 100 μL saturated Na 2 EDTA solution.
[0067] (2) SPE solid-phase extraction column (Oasis HLB column, 6mL / 500mg), pre-activated by rinsing with 5mL ethyl acetate, 5mL methanol, and 5ml pure water with pH=3.85. Turn on the vacuum pump and control the flow rate to about 3-5mL min -1 The membrane supernatant was applied to the column with a loading volume of 5 mL. After passing through the column, wash the small column with 5mL 5% methanol water and 5mL ultrapure water ...
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