Method for simultaneously separating three main components of bovine milk casein

Abstract

The invention discloses a method for simultaneously separating three main components of bovine milk casein, and aims to provide a method for separating the three main monomer components of casein, which adopts the bovine milk casein as a raw material, adopts a DEAE ion exchange column as separating equipment, and can simultaneously obtain alpha-casein, beta-casein and kappa-casein through one-timeseparation. According to the method provided by the invention, the three casein monomer components can be obtained through one-step separation, separation steps are less, a separation process is simple and easy to operate, a separation condition is mild and easy to implement, the separating equipment is simple and easy, the dosage of chemical reagents is less during a separation process, not onlyis the separation cost of the casein monomers reduced, but also the environment pollution is reduced, and the method meets a new concept on low carbon and environment protection; the average yield for separating the casein monomers is high, the average yield of the alpha-casein can reach 75 percent or more, the average yield of the beta-casein can reach 80 percent or more, and the average yield of the kappa-casein can reach 85 percent or more; the obtained three casein monomers have high purity which can reach to 95 percent or more; the method not only can meet the requirement on preparing the three high-purity casein monomer components in a laboratory, but also can be used for industrially preparing the main monomers of the casein, and is wide in application range.

Description

technical field [0001] The invention belongs to the field of protein separation and preparation, and relates to a one-time separation and preparation method of three main monomer components of casein in milk and milk products, namely α-casein, β-casein and κ-casein. It involves the application of three main components of casein in milk in food and chemical industry. Background technique [0002] The milk is degreased, acid precipitated, dehydrated, and dried to make a powder product called casein, which is a kind of protein containing phosphorus and forms micelles in milk. Casein products have good functional properties and are suitable as stabilizers, thickeners and gelling agents; and because casein has relatively concentrated hydrophilic and hydrophobic regions, it has good foaming and emulsifying properties. Casein contains 8 kinds of amino acids necessary for the human body, and can promote the absorption of calcium, phosphorus, iron, etc. by the human body, so it is w...

AI Technical Summary

Problems solved by technology

[0007] The purpose of the present invention is to provide a new type of separation process for the main monomers of bovine milk casein, to realize the one-time high-efficiency separation of three kinds of casein monomers, to solve the current Casein monomer separation process is complicated, backward technology, high cost, large consumption of chemical reagents, low recovery rate, difficult to realize industrialization and other problems

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