A rapid identification method of Phnom Penh wood turtle

Abstract

The invention discloses a method for rapid identification of Phnom Penh turtle, which can rapidly identify Phnom Penh turtle through the polymerase chain reaction of characteristic primers of Phnom Penh turtle. Using PCR method to analyze the DNA sequence of the soft-shelled turtle in Phnom Penh, find out the specific sites, and identify the soft-shelled turtle from the molecular level of the species, which can accurately, quickly and effectively distinguish the soft-shelled turtle and its confounding products of the same genus. . The rapid identification method of the Phnom Penh turtle can be simply, accurately and directly identified, and can be detected with only a small amount of dosage, and has the characteristics of high sensitivity, good stability and strong specificity, and is used in the medicinal materials of Phnom Penh turtle in the pharmaceutical process. Fast quality control.

Description

technical field [0001] The invention relates to the field of identification of medicinal materials, in particular to a rapid identification method for the Phnom Penh soil turtle. Background technique [0002] Phnom Penh Wood Beetle is the dried insect body of Opisthoplatia rientalis Burm, an insect of the family Blattaidae of the order Blattata, which has the effect of breaking blood and removing blood stasis, and strengthening tendons and bones. [0003] Due to the continuous increase of clinical use, the demand in the domestic market continues to increase, resulting in mixed fakes and inferior products, and unscrupulous businessmen shoddy the fakes. Among them, the soft-shelled soft-shelled turtle, the soft-shelled soft-shelled soft-shelled turtle, and the oriental dragonfly are the most commonly confused products. These species have similar ingredients, and after being powdered and used as medicine, it is difficult to identify them only by traditional morphological analys...

AI Technical Summary

Problems solved by technology

[0003] Due to the continuous increase of clinical use, the demand in the domestic market continues to increase, resulting in mixed fakes and inferior products, and unscrupulous businessmen shoddy and fake

Among them, the soft-shelled soft-shelled soft-shelled turtle, the soft-shelled soft-shelled soft-shelled turtle, and the oriental dragonfly are the most commonly confused products. These species have similar ingredients. After being powdered and used as medicine, it is difficult to identify them only by traditional morphological analysis.

[0004] The thin layer identification method of Phnom Penh soil tadpoles collected in the current local traditional Chinese medicinal materials is to identify its amino acid components. The dedicated properties are poor and cannot be separated from the validity of the confusing items such as the land of the land, the hills, and the Eastern submarine lice. Come

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