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A hplc method for the determination of three active components in snow gall

A technology of active ingredients and snow gall, which is applied in the field of HPLC for the determination of three active ingredients in snow gall, achieving good reproducibility, efficient and accurate method, and quality assurance

Active Publication Date: 2021-02-23
SICHUAN AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

There is no report on the simultaneous determination of three active components in Snow Gallbladder by HPLC

Method used

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  • A hplc method for the determination of three active components in snow gall
  • A hplc method for the determination of three active components in snow gall
  • A hplc method for the determination of three active components in snow gall

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Experimental program
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Embodiment 1

[0039] Embodiment 1 The inventive method measures three kinds of active ingredient contents of snow gallbladder

[0040] 1. Preparation of the test solution

[0041] The tubers of snow gallbladder are dried at 60°C, crushed, and passed through a 60-mesh sieve. Accurately weigh 1.00g of snow gall powder, put it in a round-bottomed flask, accurately pipette 25.00mL of absolute ethanol, weigh it, and after reflux extraction for 3 hours, extract it with constant temperature and ultrasonic for 60 minutes, weigh its mass, and fill up the mass with absolute ethanol , Shake well, filter through a 0.22μm microporous membrane, and obtain it.

[0042] 2. Preparation of reference solution

[0043] Accurately weigh gentianin A, gentianin B, and oleanolic acid reference substances respectively, add absolute ethanol to dissolve and constant volume, and prepare mixed reference substance solutions with mass concentrations of 0.62, 0.75, and 0.70 mg / mL respectively. spare.

[0044] 3. Estab...

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Abstract

The invention provides an HPLC method for measuring three kinds of active ingredients in snow gallbladder, the active ingredients are snow gallbladder, snow gallbladder and oleanolic acid, comprising the following steps: a, taking the snow gallbladder to be tested Powder, extract with ethanol, filter, prepare need testing solution; b, get gentianin, gentalin, oleanolic acid reference substance, mix, add ethanol to dissolve, prepare reference substance solution; c, respectively The test solution and the reference solution are injected into a high-performance liquid chromatograph for detection; d, the contents of the three components in the snow gall are calculated according to the test results. The method of the present invention can simultaneously determine the three components of gentamidin, gentamidin and oleanolic acid, the method is efficient, accurate and has good reproducibility, and provides a basis for the systematic evaluation and quality control of gentamicin, and can more comprehensively Guarantee the quality of snow gall medicinal materials.

Description

technical field [0001] The invention belongs to the technical field of chemical analysis, and in particular relates to an HPLC method for determining three active ingredients in snow gall bladder. Background technique [0002] Plants of the genus Hemsleya are perennial climbing herbaceous plants of the family Cucurbitaceae. According to Flora of my country, there are 31 species and 10 varieties of plants in this genus, which are mainly distributed in the southwest to south-central regions of China. The province holds 92% of the entire genus. The dried roots of various plants in this genus can be used for medicinal purposes. They are commonly used Chinese herbal medicines and national medicines by various ethnic groups in Southwest my country. For enteritis and various other infections, it has the advantages of less dosage, obvious effect, and no dependence. It has also been preliminarily judged as a drug that can replace antibiotics. [0003] Due to the differences in origin...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02
CPCG01N30/02
Inventor 张利杨程姜媛媛吴一超胡青青朱柏雨周永红王晓丽杨瑞武丁春邦苟丽琼佘启惠吴忠旺
Owner SICHUAN AGRI UNIV
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