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A method for hydrolyzing spider dry powder and anti-inflammatory and anti-moth toothpaste containing spider dry powder hydrolyzate

A hydrolyzate and spider technology, which is applied in the field of food and daily chemicals, can solve the problems that the product has not been seen in patents and literature reports, and achieve the effect of good dental caries, good effect and high degree of hydrolysis

Active Publication Date: 2020-04-21
HAINAN ZHUWANG PHARMA +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, it is necessary to develop a spider hydrolyzate preparation method with a high degree of hydrolysis and easy promotion and implementation. At the same time, there are no patents and literature reports on the use of spiders or their hydrolyzates as toothpaste raw materials.

Method used

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  • A method for hydrolyzing spider dry powder and anti-inflammatory and anti-moth toothpaste containing spider dry powder hydrolyzate
  • A method for hydrolyzing spider dry powder and anti-inflammatory and anti-moth toothpaste containing spider dry powder hydrolyzate
  • A method for hydrolyzing spider dry powder and anti-inflammatory and anti-moth toothpaste containing spider dry powder hydrolyzate

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0064] 2.2 Preparation of dried spider powder

[0065] 2.2.1. Pick out spiders with a body length of more than 5cm 10 days in advance for disinfection and isolation, and stop feeding to make them exhaust the excrement in their bodies. Disinfect the spider body, the breeding tank and the breeding environment with a potassium permanganate solution with a concentration of 1% every three days, and disinfect twice in total;

[0066] 2.2.2. Before harvesting spiders, prepare clean basins filled with pure water, 500mL plastic cups with lids, tweezers, washing bottles, trays and other supplies;

[0067] 2.2.3. When harvesting spiders, use tweezers to clamp the spiders out of the breeding barrel or box, put them into plastic cups, and cover them;

[0068] 2.2.4. Put the spiders into a freezer at -18°C and freeze them for 24 hours to kill them;

[0069] 2.2.5. Dry the spiders that have been frozen to death in an electric oven at a temperature of 60-70°C until the moisture content is l...

Embodiment 1

[0084] 1. Harvesting of whole insects of spiders

[0085] 1.1. Pick out spiders with a body length of more than 5cm 10 days in advance for disinfection and isolation, and stop feeding to make them exhaust the excrement in the body. Disinfect the spider body, the breeding tank and the breeding environment with a potassium permanganate solution with a concentration of 1% every three days, and disinfect twice in total;

[0086] 1.2. Before harvesting spiders, prepare clean basins filled with pure water, 500mL plastic cups with lids, tweezers, washing bottles, trays and other supplies;

[0087] 1.3. When harvesting spiders, use tweezers to clamp the spiders out of the breeding barrel or box, put them into plastic cups, and cover them;

[0088] 1.4. Put the spiders into a freezer at -18°C and freeze them for 24 hours to kill them;

[0089] 1.5. Take the frozen spider to the laboratory and put it in a clean basin filled with pure water for rinsing, and use a brush to scrub its bod...

Embodiment 2

[0112] 1. Harvesting of whole insects of spiders

[0113] With embodiment 1.

[0114] 2. Preparation of spider freeze-dried powder

[0115] With embodiment 1.

[0116] 3. Hydrolysis process of spider freeze-dried powder

[0117] 3.1 Take 10g of spider freeze-dried powder, add 250mL of purified water, stir evenly, and heat to 52°C, while using 0.1mol / L NaOH or 0.1mol / L HCl to adjust the pH to 7.2;

[0118] 3.2 Add neutral protease (enzyme activity 100,000 U / g-200,000 U / g) 9000U / g, 52°C, pH 7.2, hydrolyze for 4.2h;

[0119] 3.3 Centrifuge for 20 minutes at a speed of 5000r / min to separate hydrolyzate A and spider powder raw materials;

[0120] 3.4 Heat the hydrolyzate A to 90°C for 15 minutes to inactivate the enzyme, then cool to room temperature;

[0121] 3.5 Add 250mL of purified water to the separated spider powder raw material, stir evenly, and heat to 52°C, while adjusting the pH to 9.2 with 0.1mol / L NaOH;

[0122] 3.6 Add alkaline protease (enzyme activity 100,000 U...

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Abstract

The invention relates to the fields of food and daily chemicals, and particularly relates to a hydrolysis method of spider dry powder and anti-inflammatory and mothproof toothpaste containing hydrolysate of the spider dry powder. The hydrolysis method comprises the following steps of mixing the spider dry powder with water, adding neutral protease, carrying out hydrolysis, separating to obtain first hydrolysate and a deposit after the hydrolysis is finished, and carrying out enzyme deactivation treatment on the first hydrolysate, so as to obtain hydrolysate A; mixing the obtained deposit withwater, adding alkali protease, carrying out the hydrolysis, separating to obtain second hydrolysate after the hydrolysis is finished, and carrying out the enzyme deactivation treatment on the second hydrolysate, so as to obtain hydrolysate B; merging the hydrolysate A and the hydrolysate B, and regulating a pH (potential of Hydrogen) value to 6.5 to 7.5, so as to obtain the hydrolysate of the spider dry powder. The hydrolysis method provided by the invention is high in hydrolysis degree which reaches 51.59 percent or above; the contents of glutamic acid, methionine, aspartic acid, tyrosine andarginine in the hydrolysate are higher and the content of a small-molecule polypeptide, with bioactivity, of 1,000D to 3,000D is higher and has the proportion reaching 42.65 percent or above.

Description

technical field [0001] The invention relates to the fields of food and daily chemicals, in particular to a method for hydrolyzing dry spider powder and an anti-inflammatory and anti-moth toothpaste containing the hydrolyzed solution of dry spider powder. Background technique [0002] Toothpaste is a cleaning product commonly used in daily life and has a long history. With the continuous development of science and technology, the continuous improvement and perfection of process equipment, various types of toothpaste have come out one after another, and the quality and grade of products have been continuously improved. Now the variety of toothpaste has developed from a single clean toothpaste to a complete variety and multiple functions. , hundreds of brands of multi-functional toothpaste, to meet the needs of different levels of consumption. The common types of toothpaste are: ① Fluoride toothpaste: Fluoride can improve the corrosion resistance of teeth and inhibit the growt...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K8/98A61Q11/00A61P1/02
CPCA61K8/987A61K2800/805A61Q11/00
Inventor 朱一强李平曾青钦
Owner HAINAN ZHUWANG PHARMA
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