A fully automatic extractor for paramagnetic particles

Abstract

The invention relates to the nucleic acid extraction field, in particular to a full-automatic extractor for paramagnetic particles. The full-automatic extractor comprises a heat sealing area, a negative pressure reflux area and a nucleic acid extraction reaction area disposed between the heat sealing area and the negative pressure reflux area, the heat sealing area includes a powerful air intake fan and an air inlet cavity disposed below the powerful air intake fan, and the negative pressure reflux area includes a powerful air blower and an air outlet cavity disposed above the powerful air blower. The embodiment of the invention can provide product support for realization of reliable full-automatic high flux nucleic acid extraction in clinical practice and scientific research. And the full-automatic nucleic acid extraction does not suffer from between-sample cross contamination and amplification product contamination. The full-automatic extractor provided by the invention realizes fewconsumables and reagents used in the workflow and low cost. The full-automatic extractor provided by the invention can realize continuous sample introduction and continuous extraction of single sample, has fast extraction speed, no need to wait for batch samples, and high efficiency.

Description

technical field [0001] The invention relates to the field of nucleic acid extraction, in particular to an automatic extractor for paramagnetic particles. Background technique [0002] Nucleic acid detection is widely used in medical and health care, scientific research, inspection and quarantine and other fields, and detection technologies are also emerging one after another: PCR-ELISA, PCR-electrophoresis, PCR-hybridization, in situ hybridization, PCR real-time fluorescence, first-generation sequencing, second-generation sequencing, technologies such as third-generation sequencing. Nucleic acid extraction technology includes phenol chloroform extraction, silica fume adsorption extraction, thermal pyrolysis centrifugal extraction, membrane column extraction, magnetic bead adsorption extraction, etc. The automated nucleic acid extraction in the market includes column extraction and magnetic bead extraction. The extraction process needs to be carried out in laboratories with ...

AI Technical Summary

Problems solved by technology

[0005] 1. It must be operated in batches, and it is impossible to realize continuous sample injection at any time, and the efficiency is low;

[0006] 2. During the extraction process, the samples are all open and the samples are not isolated. Nucleic acid detection is a high-signal magnification and high-sensitivity detection. cross contamination between

The amplification detection product is a very high concentration of small molecular substances, usually with a content of more than 10 to the power of 10 copies / mL. Improper operation in the detection is likely to cause contamination of the sample by the amplification product;

[0007] 3. The extraction throughput of each batch is small and the extraction speed is slow. Generally, the column method automatically extracts up to 36 samples, and the magnetic bead method generally extracts 24, 48 or 96 samples. The extraction time is 3-10 hours, which is difficult to meet the large sample size. extraction requirements;

[0008] 4. There are biological safety hazards. At present, the automated extraction is open environment to process samples, and infectious samples cannot meet the biological safety requirements of laws and regulations during the processing process.

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