Method for directly preparing fresh bdellovibrio bacteriovorus in utilization land of bdellovibrio bacteriovorus and bdellovibrio bacteriovorus culture medium

Abstract

The invention provides a method for directly preparing fresh bdellovibrio bacteriovorus in a utilization land of the bdellovibrio bacteriovorus and a bdellovibrio bacteriovorus culture medium and belongs to the field of fermentation. The method for preparing the fresh bdellovibrio bacteriovorus by a farmer comprises the following steps: 0.5 to 15 days before the farmer needs to utilize the bdellovibrio bacteriovorus, mixing the bdellovibrio bacteriovorus culture medium containing host bacteria and water to obtain a bdellovibrio bacteriovorus culture solution; then mixing the bdellovibrio bacteriovorus culture solution with a bdellovibrio bacteriovorus strain to obtain a bdellovibrio bacteriovorus fermented solution; and standing and culturing the bdellovibrio bacteriovorus fermented solution for 12h to 72h at 18 DEG C to 36 DEG C in the presence of air. BY adopting the preparation method, a culture environment of the bdellovibrio bacteriovorus is enlarged, so that the culture of the bdellovibrio bacteriovorus is simple and easy to implement; the bdellovibrio bacteriovorus can be culture while being needed, so that a high death rate of an existing bdellovibrio bacteriovorus water product in a storage or transportation process is avoided; and meanwhile, the utilization cost of bdellovibrio bacteriovorus pure lyophilized powder also can be reduced.

Description

technical field [0001] The invention relates to the field of fermentation, in particular to a method for directly preparing fresh Bdellovibrio at the site where the Bdellovibrio is used and a Bdellovibrio culture medium. The culture medium can be liquid or powdered. Background technique [0002] Bdellovibrio is a group of bacteria that parasitize other bacteria and can cause their lysis. It is smaller than normal bacteria, can pass through bacterial filters, and acts like a bacteriophage. The strain size is 0.2~0.5μm×0.8~1.2μm. It is a natural biological control factor for the growth of pathogenic bacteria in the aquaculture water environment. It can effectively control the chemical oxygen consumption and the content of sulfide and ammonia nitrogen in the aquaculture water. New ways of biological control of aquatic animal diseases are of great significance. [0003] At present, the commercially available Bdellovibrio products in my country are composed of water and powder....

AI Technical Summary

Problems solved by technology

[0003] At present, the commercially available Bdellovibrio products in my country are composed of water and powder. The fermentation process of the water is simple, but its content is low (generally at 10 5 ~10 9 PFU / mL), the storage time is short (the content decays up to 95% after one month); and the freeze-dried powder preparation has a long storage time and is convenient for transportation, but has the disadvantage of high preparation cost. The price of pure Bdellovibrio freeze-dried powder is currently between 500 yuan / kg or more

The existing fermentation process of Bdellovibrio is cumbersome and usually needs to go through more than a dozen processes to obtain it, which is also a major reason for the high price of Bdellovibrio products

At present, the commonly used strains in aquaculture, such as lactic acid bacteria, EM bacteria, photosynthetic bacteria and Bacillus, all have their own simple culture methods, but Bdellovibrio does not, which greatly limits the application of Bdellovibrio