Saline alkali soil repairing microorganism bacterium agent and preparation method thereof

A microbial inoculant, saline-alkali soil technology, applied in the field of soil bioremediation, can solve problems such as unreasonable use of chemical fertilizers, soil ecological environment deterioration, soil nutrient imbalance, etc., and achieve good sustainable development, simple construction, and restoration of ecological balance. and productivity effects

Inactive Publication Date: 2018-03-02
MICROBIOLOGY INST OF SHAANXI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The main problems in the use of a large amount of chemical fertilizers are the unreasonable use of chemical fertilizers, the serious imbalance of nutrients in the soil, and the deterioration of the soil ecological environment, resulting in the decline of crop growth and production reduction.

Method used

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preparation example Construction

[0020] The invention relates to a preparation method of a microbial agent for remediation of saline soil, including the following steps:

[0021] Step 1: Microbial fermentation:

[0022] Adjust the pH of the liquid fermentation medium A to 7.8-8.5, and perform conventional aseptic processing, and connect with Hylococcus luteus A393 according to 8-13% of the liquid quality aseptically, at a constant temperature of 33±1℃, and a stirring speed of 120-250rpm, Ventilation volume 0.25-0.35M 3 / min, tank pressure 0.03-0.05MPa, continuous fermentation 44-50hr, microscopic examination and counting the number of cells in the fermentation broth up to (2.5-5.0)×10 9 When each / mL, according to the liquid quality 10-15% aseptic operation, access to the cold-resistant Halomonas A413 constant temperature 35 ± 1 ℃, stirring speed 180-450rpm, ventilation 0.36-0.45M 3 / min, tank pressure 0.03-0.05MPa, continuous fermentation for 88-96hr, microscopic examination and counting the number of cells in the ...

Embodiment 1

[0030] Step 1: Microbial fermentation:

[0031] Adjust the pH of the liquid fermentation medium A to 7.8, and perform routine aseptic processing. According to the aseptic operation of 13% of the liquid quality, connect with Seacoccus luteus A393, constant temperature 33±1℃, stirring speed 250rpm, ventilation volume 0.25M 3 / min, tank pressure 0.05MPa, continuous fermentation for 50hr, microscopic examination and counting of cells in the fermentation broth up to 2.5×10 9 When per mL, 15% of the liquid mass is used aseptically to access Halomonas cryospora A413 at a constant temperature of 35±1℃, stirring speed 180rpm, ventilation volume 0.45M 3 / min, tank pressure 0.05MPa, continuous fermentation for 88 hours, microscopic examination and counting the number of cells in the fermentation broth up to 5.5×10 10 The fermentation was terminated when each / mL.

[0032] Step 2: Separation of bacterial cells:

[0033] Collect the fermentation broth of Seacoccus luteus A393 and Halomonas chrysoge...

Embodiment 2

[0037] Step 1: Microbial fermentation:

[0038] Adjust the pH of the liquid fermentation medium A to 8.0, and perform conventional aseptic processing, and connect with Hylococcus luteus A393 according to 10% of the liquid quality aseptically, at a constant temperature of 33±1℃, stirring speed 200rpm, and ventilation volume 0.30M 3 / min, tank pressure 0.045MPa, continuous fermentation for 48 hours, microscopic examination and counting the number of cells in the fermentation broth up to 3.0×10 9 When each / mL, according to the liquid quality 12% aseptic operation, insert the cold-resistant Halomonas A413 constant temperature 35±1℃, stirring speed 350rpm, ventilation volume 0.4M 3 / min, tank pressure 0.04MPa, continuous fermentation for 90 hours, microscopic examination and counting the number of cells in the fermentation broth up to 6.0×10 10 The fermentation was terminated when each / mL.

[0039] Step 2: Separation of bacterial cells:

[0040] Collect the fermentation broth of Seacoccus ...

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Abstract

The invention relates to a saline alkali soil repairing microorganism bacterium agent and a preparation method thereof. In an agricultural production process, fertilizer is excessively utilized so that nutrients of agricultural products and agricultural soil are accumulated, secondary salinization is caused and certain problems on ecological security of the agricultural soil and the agricultural sustainable development are caused. In a special liquid fermentation culture medium provided by the invention, salt-tolerance microorganism micrococcus luteus A393 and cold-salt-tolerance mononucleosisA413 are cultured; a fermentation solution is collected and is centrifuged and separated to remove moisture in the fermentation solution to obtain orange red paste-shaped bacterium sludge; glycerineand kaolin are added and are uniformly stirred and mixed to obtain a white solid preparation, namely a target product. The invention provides an efficient and economical environment-friendly type saline alkali soil repairing technology; the soil saline alkali degree can be reduced by 70 percent or more; the efficient and economical environment-friendly type saline alkali soil repairing technologyhas the advantages of low treatment cost, simplicity in construction, no secondary pollution, small environment influences and the like.

Description

Technical field [0001] The invention belongs to the technical field of soil bioremediation, and specifically relates to a microbial agent for saline soil restoration and a preparation method thereof. Background technique [0002] Good soil quality plays an important role in agricultural production as an important means to ensure a bumper harvest in agriculture. The main problems in the use of a large number of chemical fertilizers are the unreasonable use of chemical fertilizers, the serious imbalance of nutrients in the soil, the deterioration of the soil ecological environment, and the decline of crop growth and production. Microbial remediation is a biological measure that utilizes the metabolic absorption, transformation, and degradation of salt-alkali ion components of specific microorganisms to achieve soil ecological balance and restoration of ecological effects. It has the characteristics of high efficiency, economy and no secondary pollution. This technology has been a...

Claims

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Application Information

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IPC IPC(8): C12N1/20B09C1/10C12R1/01
CPCB09C1/10C12N1/20
Inventor 孙晓宇陈锐李玥赵玲侠瞿佳沈卫荣
Owner MICROBIOLOGY INST OF SHAANXI
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