A compound combination for termite control and its application
A technology of termites and compositions, applied in the direction of application, resistance to vector-borne diseases, insecticides, etc., can solve problems such as damage to nerve impulse transmission, pest movement disorders, inability to eat, etc., to increase cumulative toxicity, meet environmental protection requirements, low cost effect
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Embodiment 1
[0025] Embodiment 1: the toxicity determination of single agent to termite
[0026] Determination method: Preparation of copper chloride solution: Weigh 12.8g of copper chloride in a 100mL volumetric flask, calculate according to the conversion rate of 78.1%, add distilled water to the scale line, and obtain a concentration of 100g / L copper chloride solution.
[0027] Preparation of indoxacarb solution: Weigh 10.5 g of indoxacarb into a 100 mL volumetric flask, calculate according to the conversion rate of 95%, add acetone to the scale line, and obtain a concentration of 100 g / L indoxacarb solution.
[0028] Drug film test method: Take a petri dish with a diameter of 90mm and filter paper, add 1mL of the test concentration liquid evenly on the filter paper and put it in the corresponding petri dish, then add 30 worker ants to each petri dish, Three parallels were performed at each concentration, and a blank control was set. The test was carried out under dark conditions in a ...
Embodiment 2
[0033] Embodiment 2: The toxicity determination of compound agent to termite
[0034] Assay method: the highest concentration of cupric chloride and indoxacarb used in the single-dose toxicity assay is mixed in a certain proportion into different ratios (active ingredient ratios) of binary compound (see Table 2 for the specific ratio), and then Dilute down to 3 to 5 concentrations according to the principle of equal ratio. Use a pipette to pipette 1ml of the drug solution and evenly drop it on the filter paper (Φ9cm) to form a drug film, dry it naturally and put it into a clean Petri dish (Φ9cm). Add 1ml of double-distilled water dropwise, put 30 termite worker ants in, and keep contacting and observing. 3 repetitions, set a blank control, observe and record the poisoning symptoms and death number of termites every day, and calculate the LC of various compound agents 50 value and co-toxicity coefficient, the test is carried out at 28±1°C in the dark, and keep the filter pape...
Embodiment 3
[0045] Drug film test method: Take a petri dish with a diameter of 90mm and filter paper, add 1mL of the test concentration liquid evenly on the filter paper and put it in the corresponding petri dish, then add 30 worker ants to each petri dish, Three parallels were performed at each concentration, and a blank control was set. The test was carried out under dark conditions in a constant temperature and humidity chamber (T=28±1°C, RH=80%). Record the poisoning symptoms and death number of termites every day, and keep the filter paper moist. The tested concentrations of copper chloride, indoxacarb and copper chloride: indoxacarb = 5:1 complex were 40 g / L, 400 g / L and 21 g / L, respectively.
[0046] Implementation effect: count the number of dead termites every day, and calculate the average death rate of termites after different times. The results are shown in Table 3. It can be seen that whether it is 24h, 48h, or 72h, the average mortality rate of the 21g / L compound is higher...
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