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Clean plant cellulose extraction agent, preparation method and applications thereof

A plant cellulose and extractant technology, applied in biochemical equipment and methods, microorganism-based methods, microorganisms, etc., can solve problems such as large oxygen consumption, high water consumption for chemical degumming, and environmental pollution.

Active Publication Date: 2018-02-16
晟颐天祥天然纤维科技有限公司
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  • Summary
  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, domestic and foreign cellulose extraction methods include chemical degumming method and biological degumming method. Due to the use of strong acid and strong alkali, high temperature and high pressure cooking, the chemical degumming method has complicated process, high cost, poor safety and produces a large amount of waste water. The waste water contains a large amount of Cellulose, lignin and various chemicals consume a lot of oxygen, and the discharged wastewater pollutes the environment very seriously. The large amount of wastewater discharge is one of the important sources of pollution that causes the entire water body to be polluted and the ecological environment to be destroyed.
Another kind, biological degumming has low enzyme production ability of strains, not only low degumming efficiency, but also requires high strain cultivation technology, which is still far away from industrial production
However, it solves the problems of large water consumption and serious pollution in chemical degumming

Method used

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  • Clean plant cellulose extraction agent, preparation method and applications thereof
  • Clean plant cellulose extraction agent, preparation method and applications thereof
  • Clean plant cellulose extraction agent, preparation method and applications thereof

Examples

Experimental program
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Effect test

preparation example Construction

[0075] Inoculate the Bacillus subtilis CGMCC 1.836 seed liquid into the fermenter according to the preparation method of B liquid described above, ferment at 36°C for 72 hours, and detect alkaline pectin lyase and alkaline polygalacturonic acid every 12 hours Enzyme activity and reducing sugar content, see the results figure 1 and figure 2 .

[0076] From figure 1 and 2 Visible, Bacillus subtilis (Bacillus subtilis) CGMCC 1.836 can produce alkaline pectin lyase and alkaline polygalacturonase. After 12 hours of fermentation, both enzymes were produced. With the prolongation of the fermentation period, the secretion of these two enzymes increased gradually. After 48 hours of fermentation, the enzyme activity of alkaline polygalacturonase first reached the maximum, which was 12.6U / mL. After 60 hours of fermentation, the enzyme activity of alkaline pectin lyase also reached the highest level, which was 1.4U / mL. After continuing to ferment for 72 hours, the activities of th...

Embodiment 1

[0077] The influence of embodiment 1 stabilizer on the stability of alkaline pectin lyase in B liquid

[0078] Carry out heat resistance and alkali resistance tests according to the above-mentioned methods. Determination of the residual enzyme activity rate after adding different concentrations of enzyme stabilizers, the results are shown in image 3 .

[0079] From image 3 It can be seen that the optimum concentration of adding enzyme stabilizer is 5mmol / L, at this concentration, the residual enzyme activity rate in the heat resistance test is increased by 27.6%, and the residual enzyme activity rate in the alkali resistance test is increased by 34.6%.

[0080] Four, clean cellulose extraction (degumming) process and detection method, the steps are as follows:

[0081] a. Hemp fiber degumming: open the hemp fiber, remove impurities, send it into the cooking pot, add the cooking liquid with a dilution ratio of 1:10 to 1:15, and cook it under normal pressure at 50 to 80°C f...

Embodiment 2

[0118] Example 2 Application of cellulose extractant in hemp degumming project

[0119] 1. Preparation of cooking liquid: preparation in cellulose extractant—the volume ratio of liquid A to liquid B is 5:1; then dilute with water at a ratio of 1:10 to prepare cooking liquid.

[0120] 2. Process: 5 kg of hemp fiber raw material for opening and removing impurities; liquor ratio of 1:10; adding cooking liquid and then cooking under normal pressure for 50 minutes; reaction temperature is 80°C; The test results of hemp fiber components are shown in Table 1.

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Abstract

The present invention relates to a clean plant cellulose extraction agent, a preparation method and applications thereof, and belongs to the field of biotechnology and agricultural product processingand production. The clean plant cellulose extraction agent is formed by mixing a liquid A and a liquid B, wherein a volume ratio of the liquid A to the liquid B is 3:1-5:1, the liquid A is an alkalineliquid, the liquid B comprises a crude enzyme liquid and an enzyme stabilizer, and Bacillus subtilis CGMCC1.836 is fermented, and bacterial separation is performed to obtain the clarified crude enzyme liquid. According to the present invention, the molecular structure of the colloid in the raw fiber is greatly changed by mainly using the alkaline pectin lyase, the alkaline polygalacturonase and the dilute alkali liquid, such that the stability of the colloid complex is severely damaged, and the alternating and circulating of swelling-modifying-colloid removing can be formed so as to achieve rapid hemp fiber degumming, reed pulping, cotton straw pulping and other purposes at the normal temperature.

Description

technical field [0001] The invention relates to a clean plant cellulose extractant and its preparation method and application, belonging to the fields of biotechnology and agricultural product processing and production. Background technique [0002] Plant fiber is the fiber with the largest stock, the widest application field and a long history of application on the earth. In the textile industry and papermaking industry, it has a history of thousands of years of application. At present, the domestic textile industry and papermaking industry consume nearly 100 million tons of plant fibers. Plant fiber is a supramolecular system composed of cellulose, hemicellulose, lignin, pectin, etc., with a complex structure. To realize the utilization of cellulose, it is necessary to carry out pretreatment processes such as degumming to remove part of lignin, hemicellulose, pectin, etc. Existing treatment technologies all consume a large amount of acid, alkali, water, steam, etc., whic...

Claims

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Application Information

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IPC IPC(8): C12P19/04C12R1/125
CPCC12P19/04
Inventor 季英超安家彦高晨王越张欣
Owner 晟颐天祥天然纤维科技有限公司
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