Extraction method for total flavonoids of smilacina japonica as Zhuang medicine

An extraction method and nine-story technology, applied in the field of traditional Chinese medicine analysis, can solve the problems of long extraction time, high solvent consumption, and low extraction efficiency, and achieve high extraction rate, less solvent consumption, and improved purity

Active Publication Date: 2018-02-16
GUANGXI UNIV OF CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Existing extraction methods have disadvantages such as long extraction time, high solvent consumption, and low extraction efficiency

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] The method for extracting the total flavonoids of Zhuang medicine Jiushoulou comprises the following steps:

[0029] (1) Raw material pretreatment: wash the nine-story building medicinal material, dry, pulverize, and pass through a 60-mesh sieve to obtain the nine-story building medicinal material powder;

[0030] (2) High-voltage pulse electric field extraction: add ethanol to the medicinal material powder of the nine-story building, the solid-liquid ratio of the medicinal material powder and the ethanol of the nine-story building is 1:30, soak; then carry out the high-voltage pulse electric field extraction, and the intensity of the high-voltage pulse electric field is 35kV / cm, the pulse width is 20 μs, the pulse frequency is 300 Hz, the extraction time is 30 min, filter, the filter residue is washed with ethanol, the filtrate is combined and concentrated under reduced pressure to obtain an extract;

[0031] (3) Chromatography: dissolve the extract with ethanol, add ...

Embodiment 2

[0036] The method for extracting the total flavonoids of Zhuang medicine Jiushoulou comprises the following steps:

[0037] (1) Raw material pretreatment: wash the nine-story building medicinal material, dry, pulverize, and pass through a 120-mesh sieve to obtain the nine-story building medicinal material powder;

[0038] (2) High-voltage pulse electric field extraction: add methanol to the nine-story building medicinal material powder, the solid-liquid ratio of the nine-story building medicinal material powder and methanol is 1:45, soak; then carry out high-voltage pulse electric field extraction, and the intensity of the high-voltage pulse electric field is 45kV / cm, the pulse width is 50 μs, the pulse frequency is 800 Hz, the extraction time is 10 min, filter, the filter residue is washed with methanol, the filtrate is combined and concentrated under reduced pressure to obtain an extract;

[0039] (3) Chromatography: dissolve the extract with methanol, add potassium hydroxi...

Embodiment 3

[0044] The method for extracting the total flavonoids of Zhuang medicine Jiushoulou comprises the following steps:

[0045] (1) Raw material pretreatment: wash the nine-story building medicinal material, dry, pulverize, and pass through an 80-mesh sieve to obtain the nine-story building medicinal material powder;

[0046] (2) High-voltage pulse electric field extraction: add propanol to the nine-story building medicinal material powder, the solid-liquid ratio of the nine-story building medicinal material powder and propanol is 1:40, soak; then carry out high-voltage pulse electric field extraction, the intensity of the high-voltage pulse electric field 40kV / cm, the pulse width is 30μs, the pulse frequency is 500Hz, the extraction time is 20min, filter, the filter residue is washed with propanol, the filtrate is combined and concentrated under reduced pressure to obtain the extract;

[0047] (3) Chromatography: Dissolve the extract with propanol, add sodium carbonate solution, ad...

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PUM

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Abstract

The invention provides an extraction method for total flavonoids of smilacina japonica as Zhuang medicine. The extraction method comprises the following steps: crushing a smilacina japonica medicinalmaterial, and then adding organic alcohol; extracting under a high-voltage pulse electric field and filtering; washing filter residues with the organic alcohol; combining filtrate and carrying out concentration under reduced pressure to obtain an extract; dissolving the extract, first adding an inorganic base solution and adjusting a pH value of the solution until the solution is alkaline; standing and filtering; adding an acidic water solution into the filtrate, adjusting the pH value of the solution until the solution is acidic or neutral, and carrying out natural chromatography and drying precipitate; dissolving a dried product, and filtering through a ceramic ultrafiltration membrane; and concentrating and drying the filtrate, then dissolving the filtrate with methanol, adding a sephadex column, carrying out column chromatography adsorption, and concentrating and drying an obtained yellow solution to obtain a total flavonoids pure product of the smilacina japonica. The extraction method disclosed by the invention has the advantages of simple operation, short reaction time, low consumption of the solvent and high extraction rate of the total flavonoids; and the obtained total flavonoids have high activity, and the purity of the total flavonoids is higher than 96 percent.

Description

technical field [0001] The invention belongs to the technical field of analysis of traditional Chinese medicines, and in particular relates to a method for extracting total flavonoids of Zhuang medicine nine-storied building. Background technique [0002] The Zhuang nationality in my country is mainly concentrated in Guangxi Autonomous Region. According to the investigation of relevant departments in this region, there are 709 kinds of Zhuang medicines. "Selected Works of Folk Drugs of the Zhuang Nationality" contains more than 500 commonly used Zhuang medicines. Zhuang medicines with local ethnic characteristics mainly include: Guangxi Aristolochia, Qianjinba, Dragon Boat Flower, Ginger, Carambola, Two-faced Acupuncture, Frangipani, Erythromyces chinensis, Jinjinxiang, Nanshe Bougainvillea, Ranunculus yam, Horsemane Snake, Brown House mice, pythons, etc. [0003] Nine-story building, also known as deer medicine, panlong seven, biantou seven, crab seven, white nest seven, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/896
CPCA61K36/896A61K2236/33A61K2236/51A61K2236/55
Inventor 朱华黎理王晓燕谢凤凤戴忠华张淼
Owner GUANGXI UNIV OF CHINESE MEDICINE
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