Method used for detecting alpha fetoprotein based on graphene, thionine, and nucleic acid aptamer
A technology of alpha-fetoprotein and graphene, applied in the field of detection of alpha-fetoprotein, can solve the problems of high cost, high technical requirements, complicated operation, etc., and achieve the effect of low cost, simple synthesis and stable performance
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[0040] The present invention will be described in detail below in conjunction with the accompanying drawings and specific embodiments.
[0041] A method for detecting AFP based on graphene, thionine and nucleic acid aptamer, the detection principle see figure 1 ,
[0042] The implementation steps are as follows:
[0043] 1. Electrode pretreatment: before using the screen printing electrode, put it in 0.5mol / LH 2 SO 4 In the solution, use the cyclic voltammetry scanning method to scan, the scanning voltage is -0.2 V ~ 1.0 V, and scan 10 times within this range; after the scanning is completed, the electrode is washed with distilled water and dried for later use;
[0044]2. Electrode modification and aptamer immobilization: Put the activated screen-printed electrode into 5mL0.01%HAuCl 4 In the solution, the electrodeposition was carried out while stirring with a magnetic stirrer, the deposition time was 120 s, and the deposition potential was -0.5 V. After the deposition was...
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