Rhodobacter capsulatus strain RC1 and applications thereof
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A technology of red bacteria and encapsulation, applied in the field of environmental microorganisms, can solve the problems of unclear composition and many types of activated sludge microorganisms, and achieve the effect of strong resistance
Active Publication Date: 2018-02-09
YUNNAN WAN KUI BIOLOGICAL SCI & TECH CO LTD
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The soil of cultivated plants and the sludge of paddy fields, swamps, lakes, and rivers and seas are rich in microorganisms, some of which have a formaldehyde metabolism mechanism, so many studies have tried to assemble bioreactors using sludge from potted plant soil or water, and the research results have confirmed that These bioreactors are very effective in removing and degrading various organic pollutants, but there are many types of microorganisms in the activated sludge, the composition is not clear, and some pathogenic bacteria may be contained
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Embodiment 1
[0018] Screening, identification and detection of embodiment 1 bacterial strain
[0019] The present invention first collects the bottom sludge water from the artificial wetland, adds different concentrations of formaldehyde to domesticate the microorganisms in the sludge water, and then uses the inorganic salt medium of photosynthetic bacteria to separate the photosynthetic bacteria that grow fast and have strong resistance to formaldehyde. The specific screening steps are:
[0020] 1. Domestication of activated sludge
[0021] Collect the sludge-containing water at the bottom of the artificial wetland anaerobic pool, filter it with gauze to remove large particles of impurities, add formaldehyde to the filtrate to domesticate the microorganisms in the sludge water, and the formaldehyde concentration increases from the initial 0.5mM to the final concentration every day. 10mM, continuous treatment for 20 days.
[0022] 2. Isolation of Photosynthetic Bacteria
[0023] After th...
Embodiment 2
[0028] Analysis of RC1's resistance to formaldehyde, formaldehyde removal effect and metabolic pathway
[0029] 1. Analysis of the resistance of RC1 to formaldehyde
[0030] An invention patent has been applied for a strain of capsulated rhodobacter ( Rhodobacter capsulatusstrain) DC-1 (201710038574.1), its preservation number in the China Center for Type Culture Collection is CCTCC NO: M2016676, this strain has the ability to remove formaldehyde and can be used to treat formaldehyde-contaminated sewage. In order to compare the physiological and biochemical characteristics of the strain, DC-1 and the isolated photosynthetic strain RC1 were inserted into anaerobic tubes containing 25ml of photosynthetic bacteria liquid medium, and when the OD375 of each bacteria was 0.5-0.6, respectively Add 4 mM, 6 mM formaldehyde, cultivate under light at 25°C, measure the OD375 of each bacterium at 1, 2, 3, 4, 5, 6, 7, and 8 days after inoculation, and observe the growth status of each bact...
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Abstract
The invention relates to a rhodobacter capsulatus strain RC1. The rhodobacter capsulatus strain RC1 is preserved in the China Center for Type Culture Collection, and is assigned with the accession number of CCTCC: M 2017323.The rhodobacter capsulatus is photosynthetic bacterium obtained by adopting microbes in sludge water on the bottom layer of a constructed wetland anaerobic pool domesticated byformaldehyde as a material, and carrying out separation by adopting inorganic salt culture medium of the photosynthetic bacterium, the rhodobacter capsulatus is gram negative bacterium, the bacteriumsolution is blood red, and carotenoid and bacteriochlorin a exist in bacterium cells. The rhodobacter capsulatus can grow in liquid inorganic salt culture medium containing 4 or 6 mM formaldehyde under illumination, the removal rate for formaldehyde added in the culture medium achieves 100%, formaldehyde is converted into glucose through metabolism via the photosynthetic carbon assimilation path,and the photosynthetic carbon assimilation path is the main mechanism for detoxification of formaldehyde.
Description
technical field [0001] The invention belongs to the field of environmental microbes, and in particular relates to a capsulated rhodobacter which has a formaldehyde assimilation pathway and can purify formaldehyde pollution. Background technique [0002] Formaldehyde (HCHO) is widely used in chemical synthesis, industrial manufacturing, pharmaceutical synthesis and other industrial fields. Formaldehyde also plays a pivotal role in the synthesis of chemical pesticides and their intermediates. Therefore, a large amount of liquid formaldehyde is produced in the production process of modern chemical pesticides. Produce waste water. Formaldehyde contains carbonyl groups, so it has strong electrophilic properties and very active chemical properties, and can react non-specifically with various lipids, nucleic acids and proteins, resulting in the loss of biological activity of macromolecular substances in organisms. If the production wastewater containing formaldehyde is directly di...
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