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Methoxyfluoroboron pyrrole-nucleic acid-ferric oxide complex and preparation method

A technology of methoxyfluoroboron pyrrole and ferric tetroxide, which is applied in the field of fluorescent imaging agents

Active Publication Date: 2020-07-31
JIANGSU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, both B-ultrasound and MRI are commonly used in the imaging detection of breast cancer, but both have their own limitations.

Method used

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  • Methoxyfluoroboron pyrrole-nucleic acid-ferric oxide complex and preparation method
  • Methoxyfluoroboron pyrrole-nucleic acid-ferric oxide complex and preparation method

Examples

Experimental program
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Effect test

Embodiment example 1

[0016] Implementation case 1 (best synthesis condition):

[0017] DNA 40-1 : 5'-GAGGAGACAACAACAGCGCGCGC-3' and DNA 40-1D : 5'-GCGCGCGCACAACAACAGAGGAG-3' small molecule nucleic acid as a template, the DNA 40-1 Mix with ferric chloride solution in sodium citrate buffer (pH=9, 10mM) to obtain No. 1 mixed solution, DNA 40-1 with Fe 3+ The concentrations were 6.4μM and 2000μM, and the DNA 40-1D , vitamin C solution and ferrous ammonium sulfate solution are mixed in sodium citrate buffer (pH=9, 10mM) to obtain No. 2 mixed solution, DNA 40-1D and, Vitamin C and Fe 2+The concentrations were 6.4μM, 1000μM and 1110μM respectively; then the above two mixed solutions were transferred to the water bath for heating, and the temperature rose to 67°C and stabilized for three minutes. Transfer 20 μL of ammonia water (pH=12) to a shaker for 30 minutes, then slowly cool down to room temperature for 5 hours; finally, drop 20 μL of vitamin C (10 mM) solution into the above solution, mix well...

Embodiment example 2

[0019] DNA 40-1 : 5'-GAGGAGACAACAACAGCGCGCGC-3' and DNA 40-1D : 5'-GCGCGCGCACAACAACAGAGGAG-3' small molecule nucleic acid as a template, the DNA 40-1 Mix with ferric chloride solution in sodium citrate buffer (pH=5, 10mM) to obtain No. 1 mixed solution, DNA 40-1 with Fe 3+ The concentrations were 6.4μM and 2000μM, and the DNA 40-1D , vitamin C solution and ammonium ferrous sulfate solution are mixed in sodium citrate buffer solution (pH=5, 10mM), obtain No. 2 mixed solution, DNA 40-1D and, Vitamin C and Fe 2+ The concentrations were 6.4 μM, 2000 μM and 2000 μM respectively; then the above two mixed solutions were transferred to a water bath for heating, and the temperature rose to 70°C and stabilized for three minutes, and the No. 1 and No. Transfer 20 μL of ammonia water (pH=12) to a shaker for 2 hours, then slowly cool down to room temperature for 6 hours; finally, drop 20 μL of vitamin C (10 mM) solution into the above solution, mix well and store in the dark for 5 min...

Embodiment example 3

[0021] DNA 40-1 : 5'-GAGGAGACAACAACAGCGCGCGC-3' and DNA 40-1D : 5'-GCGCGCGCACAACAACAGAGGAG-3' small molecule nucleic acid as a template, the DNA 40-1 Mixed with ferric chloride solution in sodium citrate buffer (pH=7, 10mM) to obtain No. 1 mixed solution, DNA 40-1 with Fe 3+ The concentrations were 6.4μM and 2000μM, and the DNA 40-1D , vitamin C solution and ammonium ferrous sulfate solution are mixed in sodium citrate buffer solution (pH=7, 10mM), obtain No. 2 mixed solution, DNA 40-1D and, Vitamin C and Fe 2+ The concentrations were 6.4 μM, 2000 μM and 2000 μM respectively; then the above two mixed solutions were transferred to a water bath for heating, and the temperature rose to 70°C and stabilized for three minutes, and the No. 1 and No. 2 mixed solutions were mixed evenly, and 20 μL Ammonia water (pH=12), transferred to a shaker for 2 hours, slowly cooled to room temperature, the cooling time was 6 hours; finally, 20 μL of vitamin C (10 mM) solution was added dropwi...

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Abstract

The invention relates to a fluorescent imaging agent, in particular to a methoxy boron fluoride pyrrole-nucleate-ferroferric oxide complex and a synthetic method. DNA40-1 and DNA40-1D micromolecular nucleic acid (DNA) are used as templates to synthesize novel fluorescent DNA-Fe3O4 nano particles. According to the synthesized novel fluorescent DNA-Fe3O4 nano particles, the particle size of the nanoparticles is 2 to 8 nm; an aqueous solution of the particles is dark yellow, and when excited with green light of 500nm, fluorescence is emitted at 540nm, thus showing that the methoxy boron fluoridepyrrole-nucleate-ferroferric oxide complex is a sensitive fluorescent imaging agent.

Description

technical field [0001] The invention relates to a fluorescent imaging agent, specifically a methoxyfluoroboron pyrrole-nucleic acid-ferric oxide complex and a synthesis method, belonging to the preparation and application of nucleic acid nanomaterials, specifically reporting a new type of nucleic acid-triiron tetroxide Iron complex particles loaded with methoxyboron pyrrole and applied to imaging of breast cancer cells. Background technique [0002] Cancer has long been included in the ranks of malignant diseases that threaten people's quality of life and health. If it is treated in time, the chance of cure is still very impressive. Cancer is a foreign body that cannot be recognized by the human immune system itself. It usually grows and spreads from the center of tissues and organs. Biomarkers are present in lower concentrations in early-stage cancer cells and are less detectable. Worldwide, the incidence of breast cancer ranks first among female malignant tumors. Breast ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K49/00
CPCA61K49/0019A61K49/0052
Inventor 陈秋云杨瑞
Owner JIANGSU UNIV
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