Preparation method of microbial preparation and application thereof in leather wastewater treatment
A technology for microbial preparations and leather wastewater, which is applied in microorganism-based methods, biological water/sewage treatment, animal processing wastewater treatment, etc. effect of the problem
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[0016] The present case will be further described below in conjunction with specific embodiments.
[0017] A kind of preparation method of microbial preparation, described preparation method comprises the steps:
[0018] Step 1: (1) Cultivation of lactic acid bacteria (ie, Lactobacillus fermentum and Lactobacillus casei): Take out the strains stored in the cryopreservation tube and inoculate them in 200ml of lactic acid bacteria culture solution, and culture them in a constant temperature incubator at 32°C for 48 hours. 200ml of good lactic acid bacteria was expanded to 4L, cultured at 32°C for 48 hours, and the pH value was checked after the completion of the culture. The pH value should be below 4; The strains in the storage tube were taken out and inoculated into 200ml of yeast culture solution, cultivated in a constant temperature incubator at 32°C for 48 hours, expanded the cultured 200ml of lactic acid bacteria to 4L, and cultivated at 32°C for 24 hours, and then detecte...
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