Primer and probe set and kit for detecting oncogene E6/E7 mRNA typing of high-risk human papilloma viruses

A human papillomavirus, primer probe technology, applied in the field of biochemistry, can solve problems such as low sensitivity and inaccurate detection results

Pending Publication Date: 2018-01-30
北京中生金域诊断技术股份有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] The object of the invention is to propose primers and probes and kits for detecting high-risk human papillomavirus E6/E7 mRNA typing for

Method used

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  • Primer and probe set and kit for detecting oncogene E6/E7 mRNA typing of high-risk human papilloma viruses
  • Primer and probe set and kit for detecting oncogene E6/E7 mRNA typing of high-risk human papilloma viruses
  • Primer and probe set and kit for detecting oncogene E6/E7 mRNA typing of high-risk human papilloma viruses

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Embodiment

[0036] This embodiment provides a 13 kinds of high-risk human papillomavirus mRNA detection kit, which can detect 13 high-risk human papillomavirus E6 / E7 mRNA, and the detection results can be used for auxiliary diagnosis of clinical HPV infection and cervical Early screening for cancer. This embodiment takes HPV16 as an example, and the preparation of other subtypes is the same as that of HPV16, and will not be repeated here.

[0037] Specifically follow the steps below:

[0038] 1. Query the gene sequences of 13 high-risk HPV E6 / E7 types:

[0039] Query 13 high-risk HPV E6 / E7 gene sequences through the National Center for Biotechnology Information (NCBI), where the name of HPV16 in GenBank is NC_001526.4, the corresponding base position of HPV16 E6 is 7125-7601, and the corresponding base position of HPV16 E7 At 7604-7900; HPV18 name in GenBank is AY262282., HPV18 E6 corresponds to base position 105-581, HPV18 E7 corresponds to base position 590-907; HPV31 name in GenBank ...

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Abstract

The invention relates to a primer and probe set and a kit for detecting a virus, in particular to a primer and probe set and a kit for detecting oncogene E6/E7 mRNA typing of high-risk human papillomaviruses, and belongs to the biochemical technology field. Specific upstream and downstream primers and corresponding specific molecular beacon probes are designed ingeniously for oncogenes E6/E7 of thirteen high-risk HPVs (human papilloma viruses) and reference genes beta-actin, and specific amplification and high-specificity detection of the thirteen high-risk HPVs and the reference genes beta-actin are realized. The primer and probe set and the kit can be applied to real-time quantification and typing detection of oncogene E6/E7 mRNA of the HPVs, and can be also applied to nature determination and typing detection of the thirteen HPVs at PCR (polymerase chain reaction) end points by the molecular beacon probes. The thirteen HPVs refer to HPV16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59and 68. The oncogene E6/E7 mRNA of the HPVs serves as a detection target, so that false positive results and false negative results both caused by HPV L1 DNA serving as a detection method in the prior art can be effectively avoided; the applied molecular beacon probes have the advantages of high detection sensitivity, high specificity, simplicity in operation and the like.

Description

technical field [0001] The invention relates to a primer, a probe and a kit for detecting a virus, in particular to a primer, a probe and a kit for detecting high-risk human papillomavirus E6 / E7 mRNA typing, and belongs to the technical field of biochemistry. Background technique [0002] Cervical cancer is one of the common malignant tumors in women. It is the only cancer whose cause has been found so far, which is caused by persistent infection of a single subtype of high-risk human papilloma virus (HPV). of. [0003] HPV belongs to the Papillomavacuovirus A genus of the Papovaviridae family. It is a spherical DNA virus that can cause squamous epithelial proliferation of human skin and mucous membranes. Studies have shown that more than 99% of cervical cancer is caused by HPV infection. HPV can be divided into epithelial type and mucosal type according to the infection site, among which the epithelial type includes types 1, 5, 8, 14, 20, 21, 25, 47, and the mucosal type ...

Claims

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Application Information

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IPC IPC(8): C12Q1/70C12Q1/686C12N15/11
Inventor 顾月清张万存张嘉琪
Owner 北京中生金域诊断技术股份有限公司
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