A method for pressure-controlled dry bacterial cellulose to restore the original three-dimensional network structure

A bacterial cellulose and network structure technology, applied in the field of biological materials, can solve the problems of unfavorable transportation, poor mechanical properties, complicated process, etc., and achieve the effects of easy storage and transportation, high mechanical properties and low moisture content

Active Publication Date: 2020-11-13
DONGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The freeze-drying method can better retain the original pore structure and obtain foamy bacterial nanocellulose, but the bacterial nanocellulose obtained by this dehydration method is large in size and poor in mechanical properties, which is also not conducive to transportation
Previous studies generally believed that the dry bacterial nanocellulose formed through the thermal evaporation process is difficult to restore to the original hydrogel state without chemical modification, and the process of chemical modification of bacterial nanocellulose is complicated and requires the introduction of other chemical substances, and changed the original structure of bacterial nanocellulose

Method used

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  • A method for pressure-controlled dry bacterial cellulose to restore the original three-dimensional network structure
  • A method for pressure-controlled dry bacterial cellulose to restore the original three-dimensional network structure
  • A method for pressure-controlled dry bacterial cellulose to restore the original three-dimensional network structure

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] (1) Inject the culture solution containing Gluconacetoacetobacter xylinum into the sterilized double silica gel tube reaction device (such as figure 1 shown) in the cavity of 30°C for 7 days, a BNC hydrogel tube with a length of 100mm, an inner diameter of 5mm, and an outer diameter of 10mm was obtained, which was recorded as a D-BNC tube, and then used in a water bath at 80°C The concentration was 1% NaOH alkali boiling 3 times, 4 hours each time, and repeated replacement with deionized water to neutrality to obtain the purified D-BNC tube.

[0031] (2) Put the purified D-BNC tube obtained in step (1) on a polytetrafluoroethylene tube with an outer diameter of 5 mm and a length of 100 mm, evaporate and dry the water, dry it by air blast to constant weight, and remove the polytetrafluoroethylene tube. Vinyl fluoride tubing to get dry D-BNC tubing.

[0032] (3) Connect one end of the dry D-BNC tube to the pressure gauge, and the other end to the water pump, and inject ...

Embodiment 2

[0035] (1) Inoculate the culture medium containing Gluconacetoacetobacter xylinum ATCC 23770 into a 250mL Erlenmeyer flask containing 100mL of culture medium, and culture it statically at 30°C for 7 days to obtain a BNC membrane. Boil with 1% NaOH alkali for 3 times, each time for 2 hours, and then repeatedly replace it with deionized water to neutrality to obtain a purified BNC membrane.

[0036] (2) Spread the purified BNC film obtained in step (1) on a polytetrafluoroethylene plate, perform water evaporation and drying, and blow dry to constant weight to obtain a dry BNC film.

[0037] (3) Fix the dry BNC membrane on such as Figure 5 On the device shown, DMEM (Dulbecco's Modified Eagle Medium) high-glucose medium was applied vertically on the dry BNC membrane with a pressure of 0.05 MPa, and the membrane gradually expanded. After 5 minutes, the membrane was removed to restore the three-dimensional nanofiber network structure And the DMEM high-glucose medium is filled with...

Embodiment 3

[0040] (1) Inject the nutrient solution containing Kombucha into a sterilized double silica gel tube reaction device (such as Figure 6 shown) in the cavity of 30°C for 5 days, a BNC hydrogel tube with a length of 100mm, an inner diameter of 3mm, and an outer diameter of 8mm was obtained, which was recorded as a D-BNC tube, and then used in a water bath at 80°C The concentration is 1% NaOH alkali boiling 3 times, 2 hours each time, and repeatedly replaced with deionized water to neutrality to obtain a purified D-BNC tube.

[0041](2) Put the purified D-BNC tube obtained in step (1) on a polytetrafluoroethylene tube with an outer diameter of 3 mm and a length of 100 mm, evaporate and dry the water, dry it by air blast to constant weight, and remove the polytetrafluoroethylene tube. Vinyl fluoride tubing to get dry D-BNC tubing.

[0042] (3) Connect one end of the dry D-BNC tube to the pressure gauge, and the other end to a syringe filled with blood, which is rabbit blood with ...

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Abstract

The invention relates to a method for controlling dry bacterial cellulose to recover the original three-dimensional network structure by pressure. The method comprises the following steps of obtainingBNC (bacterial nanocellulose) with the three-dimensional network structure; performing purification to obtain purified BNC; through moisture evaporation drying, obtaining dry BNC; exerting the pressure onto the dry BNC; controlling the pressure for expanding the dry BNC to obtain the BNC recovering the original three-dimensional network structure. By aiming at the characteristics that the evaporation drying BNC network structure collapse, the fluid pressure is used for exerting on the BNC, so that the BNC fast recovers the original three-dimensional network structure; important significance is realized in the fields of storage, transportation and application.

Description

technical field [0001] The invention belongs to the field of biological materials, in particular to a method for restoring the original three-dimensional network structure of dry bacterial cellulose under pressure control. Background technique [0002] Bacterial nanocellulose (BNC) is a kind of cellulose produced by bacterial secretion, which is characterized by high chemical purity, high crystallinity, high water holding capacity, three-dimensional nanofiber network structure, good biocompatibility, etc. , is a kind of biomaterial with great potential. [0003] Bacterial nanocellulose obtained by fermentation is usually a kind of hydrogel with extremely high water content, which is not convenient for storage and transportation, and dehydration and drying is a good solution. The dehydration methods of bacterial nanocellulose mainly include freeze-drying and evaporative drying. The freeze-drying method can better retain the original pore structure and obtain foamy bacterial...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08J7/02C08J7/00C08L1/02
Inventor 洪枫包露涵唐敬玉陈琳
Owner DONGHUA UNIV
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