A kind of tissue culture and in vitro regeneration method of Ting taiqing
A technology of in vitro regeneration and tissue culture, which is applied in the field of bioengineering to achieve the effect of shortening the culture time, saving the workload, and improving the quality of the seedlings
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Embodiment 1
[0022] A method for tissue culture and in vitro regeneration of Ting taiqing, the steps comprising:
[0023] (1) Disinfection of explants: select the leaves of Ting taiqing seedlings as explants, wash the soil with tap water, put it into a beaker, add water and a small amount of washing powder, make a solution for soaking, shake gently for 5min, Rinse with tap water for 2 hours, and rinse the washing powder; finally transfer to the ultra-clean workbench, disinfect with 75% ethanol solution for 3 seconds, wash with sterile water twice, and use 0.1% mercuric chloride solution containing Tween (2 drops) Disinfect for 4 minutes, wash 6 times with sterile water, and wash for later use.
[0024] (2) Induction of callus and differentiation of adventitious buds: under aseptic operation, cut the sterilized leaves into 1cm 2 Leaves of different sizes were inoculated into callus induction medium (WPM medium+TDZ2.0mg / L+NAA 0.2mg / L+agar 4.5g / L+sucrose 20g / L, pH=5.8) During the process, m...
Embodiment 2
[0028] A method for tissue culture and in vitro regeneration of Ting taiqing, the steps comprising:
[0029] (1) Disinfection of explants: select the leaves of Ting taiqing seedlings as explants, wash the soil with tap water, put it into a beaker, add water and a small amount of washing powder, make a solution for soaking, shake gently for 5min, Rinse again with tap water for 2 hours, and rinse off the washing powder; finally transfer to the ultra-clean workbench, disinfect with 75% ethanol solution for 5 seconds, wash with sterile water twice, and use 0.1% mercuric chloride solution containing Tween (2 drops) Disinfect for 5 minutes, wash with sterile water for 6 times and set aside;
[0030] (2) Induction of callus and differentiation of adventitious buds: under aseptic operation, cut the sterilized leaves into 1.2cm 2size, inoculated into the callus induction medium (MS medium + TDZ 1.5mg / L + NAA0.1mg / L + agar 4.5g / L + sucrose 20g / L, pH = 5.8) in the way that the leaves ar...
Embodiment 3
[0034] A method for tissue culture and in vitro regeneration of Ting taiqing, the steps comprising:
[0035] (1) Disinfection of explants: select the leaves of Ting taiqing seedlings as explants, wash the soil with tap water, put it into a beaker, add water and a small amount of washing powder, make a solution for soaking, shake gently for 5min, Rinse again with tap water for 2 hours, and rinse off the washing powder; finally transfer to the ultra-clean workbench, disinfect with 75% ethanol solution for 3 seconds, wash with sterile water twice, and disinfect with 0.1% mercuric chloride solution containing Tween (2 drops) 4min, wash 6 times with sterile water, wash and set aside;
[0036] (2) Induction of callus and differentiation of adventitious buds: under aseptic operation, cut the sterilized leaves into 1.5cm 2 Size, inoculated into callus induction medium (1 / 2MS medium + TDZ 2.5mg / L + NAA 0.3mg / L + agar 4.5g / L + sucrose 20g / L, pH = 5.8) In the medium, make the incision ...
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