Method for detecting hydrogen peroxide by modifying screen printing electrode by persimmon tannin-graphene-Pt composite material

Abstract

The invention discloses a method for detecting hydrogen peroxide by modifying a screen printing electrode by a persimmon tannin-graphene-Pt composite material. The method is characterized in that persimmon tannin is taken as a raw material, a one-step reduction method is used for directly preparing the persimmon tannin-graphene-Pt nano composite material, an electrodeposition technology is employed for depositing nano-gold on a surface of the screen printing electrode, the persimmon tannin-graphene-Pt nano composite material dropped on the electrode surface is absorbed to the surface of the screen printing electrode through electrostatic adsorption, adsorption of persimmon tannin on metal ions, large specific surface area of graphene, and high efficiency catalysis of nano Pt and catalysisof H2O2 are used, a H2O2 non-enzymatic sensor is constructed, and an electric current-time method is employed for timing current response detection of H2O2. A work curve is drafted according to a relation of current response of the sensor and H2O2 concentration, and quantitative determination of H2O2 is realized. The method has the advantages of good stability, high specificity, and easy, convenient and rapid operation.

Description

technical field [0001] The invention belongs to the technical field of biological detection, and in particular relates to a method for detecting hydrogen peroxide by a composite material modified screen printing electrode. Background technique [0002] Intracellular hydrogen peroxide (H 2 o 2 ) content and its dynamic release process can elucidate the mechanism of the signal transduction pathway, and have a deeper understanding of cell aging and apoptosis, which is conducive to expanding its application in clinical pathological diagnosis. Currently used to detect H 2 o 2 The techniques mainly include spectrophotometry, colorimetry, fluorescence, chromatography, and chemical methods. The invention patent with the publication number CN105692719A discloses a colorimetric method for detecting hydrogen peroxide through cobalt sulfide nanomaterials, adding cobalt sulfide phosphate buffer solution into Tirs-HCl buffer solution, and then adding the solution to be detected, TMB ...

AI Technical Summary

Problems solved by technology

[0003] The technical problem to be solved by the present invention is to provide a kind of utilizing persimmon tannin-graphene-Pt composite material to modify the screen printing electrode to detect H by electrochemical method. 2 o 2 In order to solve the problems of current enzyme biosensors such as susceptibility to environmental influence, poor conductivity and high technical requirements

Images