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Method for rapidly detecting veterinary antibiotics

A technology for veterinary antibiotics and antibiotics, which is applied in the direction of analyzing materials through chemical reactions and analyzing materials through observation of the impact on chemical indicators, which can solve the problems of increasing economic losses, increasing prevention and control, overdose or misuse of drugs to save time and cost, reduce economic losses, and promote the growth of germs

Inactive Publication Date: 2018-01-19
凤阳高科动物保健品厂
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Many farmers are limited by their technical level and conditions of use, and lack correct judgment on the selection of effective antibacterial drugs. Blind drug use is more common, resulting in at least the following consequences: failure to prescribe the right drug, delaying the timing of prevention and treatment; excessive drug use or misuse, increasing unnecessary economic losses The drug resistance of pathogenic bacteria is enhanced, which will increase the difficulty of further prevention and control; unnecessary drug residues in animals will occur, which will damage the health of consumers. The test is to borrow human drug sensitivity test paper, which has disadvantages such as long cycle, inconvenient use, high price, and easy deviation of results, so it cannot be used socially; we selected nearly 20 kinds of antibiotics commonly used by veterinarians, and developed a drug sensitivity test kit for livestock and poultry ;It has the advantages of simple operation, no need for any equipment, fast and reliable results, no special training, etc.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Sick pig detection:

[0021] On August 7, 2017, a sick pig from a professional pig raising household in Zhongzhuang Village, Xiaoxi Town, Fengyang County was treated.

[0022] (1) Sampling: Open the test box, take out the test plate, sampling dropper, sampling cotton swab, instruction manual, two bags of heating pads, use the cotton swab to collect the diseased mucus of sick pigs, pull out the No. 22 tube according to the number on the test plate, and open the tube Cover, and insert the sampled cotton swab into the tube for elution for 1 minute to obtain the bacteria-containing solution;

[0023] (2) Adding samples: Use a sample dropper to absorb the bacteria-containing solution in the shaken No. 22 tube, take out the No. 1 tube according to the test plate number, open the tube cap, drop 1 drop of the bacteria-containing solution, close the tube cap tightly, and shake After uniformity, insert it back into the No. 1 hole, and then operate the No. 2~20 tubes sequentially...

Embodiment 2

[0028] Sick Chicken Detection:

[0029] On June 13, 2017, the sick chicken of Guo, a professional chicken farmer in Huanglou Village, Majing Town, Suzhou County, was treated.

[0030] (1) Sampling: Open the test box, take out the test plate, sampling dropper, sampling cotton swab, instruction manual, and two bags of heating pads, cut the diseased organ liver of a dead chicken into a "ten"-shaped deep opening, and insert the cotton swab into the deep mouth. Dip the lesion liquid in the mouth, pull out the No. 22 tube according to the number on the test plate, open the tube cover, insert the sampled cotton swab into the tube for elution for 1 minute, and obtain the bacteria-containing solution;

[0031] (2) Adding samples: Use a sample dropper to absorb the bacteria-containing solution in the shaken No. 22 tube, take out the No. 1 tube according to the test plate number, open the tube cap, drop 1 drop of the bacteria-containing solution, close the tube cap tightly, and shake Af...

Embodiment 3

[0036] Sick sheep detection:

[0037] On May 11, 2017, the sick sheep of Han, a professional sheep raising household in Lianglou Village, Tiefo Town, Suixi County, were treated.

[0038] (1) Sampling: Open the test box, take out the test plate, sampling dropper, sampling cotton swab, instruction manual, and two bags of heating pads, cut the lung of the diseased organ of the dead sheep into a "ten" deep opening, and insert the cotton swab into the deep mouth. Dip the lesion liquid in the mouth, pull out the No. 22 tube according to the number on the test plate, open the tube cover, insert the sampled cotton swab into the tube for elution for 1 minute, and obtain the bacteria-containing solution;

[0039] (2) Adding samples: Use a sample dropper to absorb the bacteria-containing solution in the shaken No. 22 tube, take out the No. 1 tube according to the test plate number, open the tube cap, drop 1 drop of the bacteria-containing solution, close the tube cap tightly, and shake ...

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PUM

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Abstract

The invention mainly relates to the field of animal husbandry and veterinary medicines and discloses a method for rapidly detecting veterinary antibiotics. The method comprises the following steps: taking samples, feeding the samples, culturing the samples, and performing result judgment. A bacterial liquid is directly prepared after the sample is taken, then the activity of bacteria in a diseasematerial is maintained, the culture time is shortened to 16 hours, effective treatment on sick livestock is accelerated, and the economic loss of farmers is reduced; the method is simple and convenient, no professional skill or large-size equipment is needed, heat-preservation culture is implemented after the sample is taken, and the testing time is shortened; if growth of pathogenic bacteria cannot be inhibited by antibiotics in a testing tube, the testing tube turns into red from clear colorless, the conventional operation that the size of an inhibition zone is observed is replaced by observation on color change, relatively good sensitivity is achieved, the operation is simple and visible, antibiotics can be accurately selected by farmers, appropriate medicines can be administrated for diseases accurately, diseases can be prevented and treated rapidly, and the economic loss of the farmers is reduced; complete types of antibiotics can be put into the testing tube, pathogenic bacteriaof livestock can be detected, and thus the method is wide in application range.

Description

technical field [0001] The invention mainly relates to the field of animal husbandry and veterinary medicine, in particular to a method for rapidly detecting veterinary antibiotics. Background technique [0002] The abuse of antibiotics in the prevention and control of animal diseases and the emergence of a large number of drug-resistant strains have become serious problems. Many farmers are limited by their technical level and conditions of use, and lack correct judgment on the selection of effective antibacterial drugs. Blind drug use is more common, resulting in at least the following consequences: failure to prescribe the right drug, delaying the timing of prevention and treatment; excessive drug use or misuse, increasing unnecessary economic losses The drug resistance of pathogenic bacteria is enhanced, which will increase the difficulty of further prevention and control; unnecessary drug residues in animals will occur, which will damage the health of consumers. The te...

Claims

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Application Information

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IPC IPC(8): G01N21/78
Inventor 周岩
Owner 凤阳高科动物保健品厂
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