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Regulation and control method for promoting directional degradation of organic pollutants through compost humus reducing bacteria

A technology for organic pollutants and humus, which is applied in the methods of using microorganisms, the preparation of organic fertilizers, organic fertilizers, etc., can solve problems such as the difficulty of reducing bacteria in humus, and achieve remarkable control effects, clear control steps, and improved operability. Effect

Inactive Publication Date: 2018-01-19
CHINESE RES ACAD OF ENVIRONMENTAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the large number and types of humus-reducing bacteria produced during the composting process, identifying the humus-reducing bacteria that play a significant role in PCP degradation is like looking for a needle in a haystack, and it is particularly difficult to obtain key humus-reducing bacteria using traditional isolation, purification and culture techniques

Method used

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  • Regulation and control method for promoting directional degradation of organic pollutants through compost humus reducing bacteria
  • Regulation and control method for promoting directional degradation of organic pollutants through compost humus reducing bacteria
  • Regulation and control method for promoting directional degradation of organic pollutants through compost humus reducing bacteria

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Experimental program
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Effect test

Embodiment 1

[0034] Take chicken manure (CM), weeds (WW), dead branches (GW) and other composting materials for composting, collect samples of compost in three stages of heating period, high temperature period and decomposing period, enrich and cultivate humus reducing bacteria and use them The flora structure was detected by 16S rDNA sequencing. The test results are shown in Table 1 (CM1, CM2 and CM3 represent the samples of chicken manure composting in the heating period, high temperature period and decomposing period respectively, and the rest are similar) and Table 2. The different physical and chemical indicators are shown in Table 3, including temperature, water content, ammoniacal nitrogen (NH 4 + -N), nitrate nitrogen (NO 3 - -N), water soluble organic nitrogen (DON), water soluble organic carbon (DOC), C / N, organic matter (OM) and pH.

[0035] Table 1 The sum of the sequence numbers of humus-reducing bacteria at the genus level in the composting process of different materials ...

Embodiment 2

[0055] Cow manure (DCM), fruits and vegetables (FVW), straw (SW) and sludge (SS) were taken as compost materials for composting, and the samples of compost in the three stages of heating up period, high temperature period and decomposing period were collected respectively to enrich and cultivate humus The bacteria were reduced and the flora structure was detected by 16S rDNA sequencing. The test results are shown in Table 2 and Table 4. The different physical and chemical indicators are shown in Table 5, including temperature, water content, NH 4 + -N, NO 3 - -N, DON, DOC, C / N, OM and pH.

[0056] Table 4 The sum of the sequence numbers of humus-reducing bacteria at the genus level in the composting process of different materials

[0057]

[0058]

[0059]

[0060]

[0061] Table 5 Changes of microenvironmental factors during composting of cow dung, fruits and vegetables, straw and sludge

[0062]

[0063]

[0064] Humus-reducing bacteria enriched in dif...

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Abstract

The invention discloses a regulation and control method for promoting directional degradation of organic pollutants through compost humus reducing bacteria. The regulation and control method comprisesthe following steps: enriching humus reducing bacteria in different compost stages, carrying out 16Sr RNA sequencing on the humus reducing bacteria, and measuring the microenvironment factors in thecompost process; carrying out biological degradation on the organic pollutants in anaerobic conditions, and acquiring key humus reducing bacteria capable of promoting the degradation of the organic pollutants by utilizing canonical correspondence analysis; and finally providing the regulation and control method by utilizing the key parameters influencing the key humus reducing bacteria by utilizing the canonical correspondence. Compared with the prior art, the method has the advantages that the degradation of the organic pollutants is promoted by a method for directionally regulating and controlling the key humus reducing bacteria, has the characteristics of strong pertinence, simple method, strong controllability and the like, and can provide reliable technical support for repairing soilpolluted by the organic pollutants through compost in the future.

Description

technical field [0001] The invention relates to the field of biodegradation of organic pollutants, in particular to a control method for promoting the directional degradation of organic pollutants by compost humus-reducing bacteria. Background technique [0002] The extensive use of organic matter has caused worldwide soil and water pollution, and the residues of organic matter pollutants pose a great threat to human health and the ecological environment. For example, since the 1930s, pentachlorophenol (PCP) has been It is widely used as wood preservative, fruit and vegetable insecticide or fungicide. PCP is relatively stable in nature, not easy to be oxidized and hydrolyzed under normal conditions, and is the most difficult to degrade phenolic substance. At the same time, PCP is the most toxic among phenols and has strong carcinogenic, teratogenic and mutagenic effects. In soil rich in organic matter, PCP is more easily absorbed by plants, and its volatility is very low. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/02C12N1/36C05F17/00
CPCY02W30/40
Inventor 赵昕宇席北斗何小松张慧党秋玲檀文炳崔东宇
Owner CHINESE RES ACAD OF ENVIRONMENTAL SCI
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