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Method for extracting chlorogenic acid and scopoletin from tobacco leaf

A technology of scopolamine and chlorogenic acid is applied in chemical instruments and methods, separation/purification of carboxylate, preparation of carboxylate, etc. The effect of improving utilization

Active Publication Date: 2018-01-19
HUNAN AGRICULTURAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The backlog of tobacco leaves is more serious, and there is no method to extract chlorogenic acid and scopolamine from the upper tobacco leaves

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Weigh 100kg tobacco leaf raw material, add 450L petroleum ether after pulverization, reflux extraction for 2.5h, filter with ceramic membrane to obtain filtrate I and filter residue I, filtrate I evaporate to dryness under reduced pressure and recover petroleum ether to obtain 4.53kg extract I, use 25L ethyl acetate Extract and concentrate under reduced pressure to obtain 2L ethyl acetate extract I.

[0030] Add 300L of pH 2 water to filter residue I, stir and extract at room temperature for 5 hours, filter with ceramic membrane to obtain 240L of filtrate II and filter residue II, add 250L of 65% ethanol solution to filter residue II, stir and extract at 60°C for 3 hours, and obtain 240L of filtrate III and filter residue III through ceramic membrane filtration The filtrate III was concentrated under reduced pressure until there was no ethanol smell to obtain 60L concentrated solution I, which was then combined with the filtrate II, extracted with 1500L ethyl acetate, an...

Embodiment 2

[0033] Weigh 200kg of raw tobacco leaves, crush them, add 1000L of petroleum ether, reflux and extract for 3 hours, filter with a ceramic membrane to obtain filtrate I and filter residue I, filtrate I evaporate under reduced pressure to recover petroleum ether to obtain 9.24kg of extract I, and extract with 80L of ethyl acetate , and concentrated under reduced pressure to obtain 4 L of ethyl acetate extract I.

[0034] Add 700L of water with pH 1.5 to filter residue I, stir and extract at room temperature for 4 hours, filter through ceramic membrane to obtain 600L of filtrate II and filter residue II, add 500L of 70% ethanol solution to filter residue II, stir and extract at 55°C for 6 hours, and obtain 480L of filtrate III and filter residue II through ceramic membrane filtration Filter residue III; filtrate III was concentrated under reduced pressure until there was no ethanol smell to obtain 100L concentrated solution I, which was then combined with filtrate II, extracted wi...

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PUM

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Abstract

The invention relates to a method for extracting chlorogenic acid and scopoletin from tobacco leaf. The method comprises the following steps: extracting a tobacco leaf raw material with a petroleum ether, filtering, performing evaporating to dryness under reduced pressure so as to obtain extract, extracting with ethyl acetate to obtain an extracting solution, adding water into filter residues andextracting with ethanol, extracting the filtrate obtained by filtration with ethyl acetate, performing vacuum concentration to obtain an extracting solution, mixing the extracting solution, loading onto a silica gel column of 80-100 meshes, respectively eluting with water and ethyl acetate, performing vacuum concentration, crystallizing and recrystallizing so as to obtain the products chlorogenicacid and scopoletin, carbonizing filter residues at a temperature of 150-180 DEG C, and pelletizing to obtain a carbide so as to be directly used as an adsorbent. According to the method disclosed bythe invention, the chlorogenic acid and scopoletin can be synchronously extracted, the utilization rate of the tobacco leaf is effectively improved, and the production cost is reduced. By utilizing water extraction, alcohol extraction and petroleum ether extraction, most of substances in the tobacco leaf are removed, so that the extracted wastes can be used for preparing adsorbing materials, and the yield of the chlorogenic acid and scopoletin is improved.

Description

Technical field: [0001] The invention belongs to the technical field of natural product processing, and relates to a method for extracting chlorogenic acid and scopolamine from tobacco leaves. Background technique [0002] Nicotiana is an annual or limited perennial herb of the family Solanaceae, with a slightly lignified base. Inflorescences terminal, paniculate, many-flowered; capsules ovate or oblong, about as long as persistent calyx. The average contents of chemical components in flue-cured tobacco are: 23.08% of total sugar, 19.63% of reducing sugar, 2% of total nitrogen, 1.99% of nicotine, 10.32% of protein, Schmuck value 2.45, total sugar / nicotine ratio 14.21, total nitrogen / nicotine ratio 1.15. It also contains functional ingredients such as rutin, solanesol, and chlorogenic acid. Tobacco is warm in nature, sweet in taste, poisonous, and has the effects of reducing swelling, detoxifying, and killing insects. It is mainly used for boils, tinea capitis, tinea alba...

Claims

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Application Information

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IPC IPC(8): C07C69/732C07C67/48C07C67/58C07C67/56C07C67/52C07D311/16B01J20/20
Inventor 周喜新
Owner HUNAN AGRICULTURAL UNIV
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