Method for researching analgesic and anti-inflammation effects of fallopia aubertii
An analgesic, anti-inflammatory and effective technology, applied in the field of medicine, can solve the problems of not finding the analgesic and anti-inflammatory effects of Polygonum vulgaris
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[0037] The technical solutions of the present invention will be further described in detail below in conjunction with specific embodiments.
[0038] 1 Analgesic effect research
[0039] 1.1 Materials
[0040] 1.1.2 Drugs
[0041] Polygonum vine stem extract freeze-dried powder. Aspirin effervescent tablets (AstraZeneca Pharmaceutical Co., Ltd., batch number 1401102). Glacial acetic acid (analytical grade), normal saline.
[0042]
[0043] 1.1.3 Instruments
[0044]
[0045] 1.2 Method
[0046] 1.2.1 Effects on pain induced by heat stimulation in mice
[0047] (1) Adjust the constant temperature
[0048] Intelligent hot plate instrument set temperature (55.0±0.5) ℃, preheat for 10min.
[0049] (2) Select qualified mice
[0050] Take healthy female mice, weighing 18-22 g, and place one mouse on the hot plate apparatus each time. The pain threshold of the mouse is defined as the time (s) required for the mouse to lick the hind paw after placing the hot plate appara...
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